CD16 Antibody | 2H7

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CD16 Antibody | 2H7 gallery image 1

Immunoperoxidase staining of formalin fixed, paraffin embedded human lung with Mouse anti Human CD16 antibody (MCA1816) following heat mediated antigen retrieval using citrate buffer, pH6.0. Low power

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CD16 Antibody | 2H7 gallery image 2

Immunoperoxidase staining of formalin fixed, paraffin embedded human lung with Mouse anti Human CD16 antibody (MCA1816) following heat mediated antigen retrieval using citrate buffer, pH6.0. High power

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Published customer image:
Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Distribution of CD4+ and CD16+ cells in squamous epithelia of the oral and ectocervical mucosae and in simple columnar epithelia of the rectum/sigmoid and endocervix. Tissue sections were stained by standard IHC for CD4 (Panels A,C,E,G) or CD16 (Panels B,D,F,H), as described in Materials and Methods. Representative images are shown. Receptor-positive cells appear brown and cell nuclei appear blue. Arrows indicate a few examples of positive cells located within the epithelium. Small round CD4bright cells (black arrows) are T cells, while antigen-presenting cells (APC) are larger and CD4dim (yellow arrows). Note that CD4+ cells are typically present several cell layers deep within the stratified squamous epithelium and lamina propria of oral (A) and ectocervical mucosae (C), similar to CD16+ APC (B and D, respectively). Small round CD4bright lymphocytes, CD4dim APC and CD16+ APC are also present within the simple columnar epithelia (E-H). Note the horizontal orientation of CD16+ APC at the base of columnar cells in the endocervix (F). Also note that some CD16+ APC in the rectal epithelium are oriented vertically with dendritic processes reaching the luminal surface (insert, H). Bar = 100 μm.

From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z (2015) Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.
PLoS ONE 10(7): e0132942.

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CD16 Antibody | 2H7 gallery image 4

Published customer image:
Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Examples of co-localization of CD16+ cells (top row) and gp340 expression (bottom row) in columnar epithelia of the colon/rectum. Serial 5μm sections were stained by IHC for gp340 and CD16, as described in Materials and Methods. The three CD16/gp340 pairs of images represent samples from three different subjects, and each pair is from the same site of the specimen. Gp340 and CD16 are stained brown (some examples marked with arrows), and cell nuclei are blue. Note intraepithelial CD16+ cells and brown granular staining of gp340 throughout the non-mucous columnar cells at the same sites.

From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z (2015) Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.
PLoS ONE 10(7): e0132942.

Enlarge
CD16 Antibody | 2H7 gallery image 5

Published customer image:
Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Examples of the distribution of CD4+ cells and gp340 expression in ectocervical stratified squamous (A, B) and endocervical columnar (C, D) epithelia. Serial 5μm sections were stained by IHC for gp340 and CD16, images from the same area of the specimens are shown. Gp340 and CD16 are stained brown (some examples marked with arrows), and cell nuclei are blue. Note the presence of CD4+ cells the brown granular pattern of gp340 staining throughout the ectocervical squamous cells of the spinous layer above the basal undifferentiated keratinocytes, as well as in the majority of the endocervical columnar epithelial cells. Also note the distribution of CD4+ cells among the columnar epithelial cells at the same location.

From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z (2015) Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.
PLoS ONE 10(7): e0132942.

Enlarge
CD16 Antibody | 2H7 gallery image 6

Published customer image:
Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Interface between the epithelium and the underlying lamina propria is identifiable on the basis of morphology. Sections of colon/rectum (A and B) and of endocervix (C and D) were stained by standard H&E (top panels) or by IHC for CD16. The interface between the epithelium and the lamina propria is indicated with black arrows. CD16+ cells are stained brown. Note many intra-epithelial CD16+ cells (above the basement membrane).

From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z (2015) Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.
PLoS ONE 10(7): e0132942.

Enlarge
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  • Mouse anti Human CD16
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  • Product Type
    Monoclonal Antibody
  • Clone
    2H7
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1816TP *datasheet pdfdatasheet pdf0.1 ml
    MCA1816T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD16 antibody, clone 2H7 recognizes the human Low affinity immunoglobulin gamma Fc region receptor III, also known as CD16. CD 16 exists as both a transmembrane (CD16a) and a GPI-anchored (CD16b) cell surface antigen expressed by granulocytes, NK cells, macrophages and a subset of T-cells. Mouse anti Human CD16 antibody, clone 2H7 recognizes both forms of the CD16 molecule.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)1/201/40
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Tonsil
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD16 Antibody Formats

    Formats Clone Applications Sizes available
    CD16 Antibody : S/N 2H7 P * 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Tonsil
              • Application NameReference Images
                Immunohistology - Paraffin

              References

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