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Mouse anti Human CD16 antibody, clone 2H7 used for the demonstration of CD16 expressing cells in epithelia by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Distribution of CD4+ and CD16+ cells in squamous epithelia of the oral and ectocervical mucosae and in simple columnar epithelia of the rectum/sigmoid and endocervix. Tissue sections were stained by standard IHC for CD4 (Panels A,C,E,G) or CD16 (Panels B,D,F,H), as described in Materials and Methods. Representative images are shown. Receptor-positive cells appear brown and cell nuclei appear blue. Arrows indicate a few examples of positive cells located within the epithelium. Small round CD4bright cells (black arrows) are T cells, while antigen-presenting cells (APC) are larger and CD4dim (yellow arrows). Note that CD4+ cells are typically present several cell layers deep within the stratified squamous epithelium and lamina propria of oral (A) and ectocervical mucosae (C), similar to CD16+ APC (B and D, respectively). Small round CD4bright lymphocytes, CD4dim APC and CD16+ APC are also present within the simple columnar epithelia (E-H). Note the horizontal orientation of CD16+ APC at the base of columnar cells in the endocervix (F). Also note that some CD16+ APC in the rectal epithelium are oriented vertically with dendritic processes reaching the luminal surface (insert, H). Bar = 100 μm.
From: Patyka M, Malamud D, Weissman D, Abrams WR, Kurago Z (2015) Periluminal Distribution of HIV-Binding Target Cells and Gp340 in the Oral, Cervical and Sigmoid/Rectal Mucosae: A Mapping Study.
PLoS ONE 10(7): e0132942.