Figure A. Unstimulated cells stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and PE conjugated Mouse anti Human CD154 (MCA1938PE). Figure B. Cells stimulated with PMA and ionomycin for 5 hr then stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and PE conjugated Mouse anti Human CD154 (MCA1938PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.
Rat anti Human CD154 antibody, clone YMF323.6.2 recognizes the human CD154 cell surface antigen, a 32kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells. Rat anti Human CD154 antibody, clone YMF323.6.2 also recognizes the soluble form of CD154.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitute with 1.0 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. After reconstitution store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul