CD154 Antibody | YMF323.6.2

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CD154 Antibody | YMF323.6.2 gallery image 1

Figure A. Unstimulated cells stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and PE conjugated Mouse anti Human CD154 (MCA1938PE). Figure B. Cells stimulated with PMA and ionomycin for 5 hr then stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 (MCA463A700) and PE conjugated Mouse anti Human CD154 (MCA1938PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer.

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CD154 Antibody | YMF323.6.2 gallery image 2

Recombinant Human CD154 (PHP126) detected with Rat anti Human CD154 (MCA1938)

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  • Rat anti Human CD154:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YMF323.6.2
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1938PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1938PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Human CD154 antibody, clone YMF323.6.2 recognizes the human CD154 cell surface antigen, a 32kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells. Rat anti Human CD154 antibody, clone YMF323.6.2 also recognizes the soluble form of CD154.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD154 Antibody Formats

    Formats Clone Applications Sizes available
    CD154 Antibody : RPE YMF323.6.2 F 100 Tests
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:RPEMCA6005PE100 TestsF
        MCA6005PE

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

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