CD154 Antibody

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Staining of formalin fixed, paraffin embedded human cervical squamous cell carcinoma with Goat anti Human CD154 (AHP1029)

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Staining of formalin fixed, paraffin embedded human cervical squamous cell carcinoma with Goat anti Human CD154 (AHP1029)

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  • Goat anti Human CD154:Biotin
  • Goat anti Human CD154
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1029E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1029
    AHP1029BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP1029B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human CD154 antibody recognizes the soluble form of human CD154, also designated CD40L (sCD40 Ligand), a member of the TNF receptor superfamily, comprising the full-length of the TNF homologous region of CD154.

      CD154 is expressed predominantly on activated CD4+ T lymphocytes, and acts as a costimulatory molecule in B cell differentiation/proliferation, immunoglobulin switching and germinal centre formation, through interaction with the CD40 receptor on B cells, and cytokines secreted by activated T cells.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Yeastyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to human CD154 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present.
      None present.
    • Immunogen
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50ug/ml
      IgG concentration 1.0mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      inflammatory response
      leukocyte cell-cell adhesion
      platelet activation
      isotype switching
      CD40 receptor binding
      B cell proliferation
      cytokine activity
      tumor necrosis factor receptor binding
      anti-apoptosis
      integral to plasma membrane
      soluble fraction
      extracellular space
      positive regulation of interleukin-12 production
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.150.30ug/ml
      Western Blotting0.10.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)2.55.0ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated human CD154 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CD154 antibody (AHP1029) as the capture reagent and recombinant human CD154 (PHP126) as the standard.
    • ELISA
      Purified goat anti human CD154 may be used in an indirect ELISA or as a capture reagent in a sadwich ELISA with our biotinylated human CD154 antibody (AHP1029B) as the detection reagent and recombinant human CD154 (PHP126) as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Human cervical squamous cell carcinoma
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD154 Antibody Formats

    Formats Applications Sizes available
    CD154 Antibody : Biotin E, WB 50 µg
    CD154 Antibody : Purified E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human cervical squamous cell carcinoma

              Further Reading

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