Mouse anti Human CD150 antibody, clone A12 recognizes human CD150, a 70 kDa cell surface glycoprotein also known as signalling lymphocytic activation molecule (SLAM). CD150 is constitutively expressed on CD45+ ve T-cells, immature thymocytes, a subpopulation of peripheral B-cells and dendritic cells. CD150 is rapidly upregulated in T-cells, B-cells and dendritic cells after activation. In addition to the membrane form of CD150, activated T and B cells also express mRNA encoding a secreted form of CD150. Mouse anti Human CD150 antibody, clone A12 is reported to induce proliferation of pre-activated T cells (Cocks et al. 1995).
N.B. Antibody reactivity and working conditions may vary between species.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
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