CD150 Antibody | A12

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CD150 Antibody | A12 gallery image 1

Staining of human peripheral blood monocytes with Mouse anti Human CD150 (MCA2251)

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CD150 Antibody | A12 gallery image 2

Staining of human peripheral blood monocytes with Mouse anti Human CD150:FITC (MCA2251F)

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  • CD150 Antibody | A12 thumbnail image 1
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  • Mouse anti Human CD150:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    A12
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2251FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2251F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD150 antibody, clone A12 recognizes human CD150, a 70 kDa cell surface glycoprotein also known as signalling lymphocytic activation molecule (SLAM). CD150 is constitutively expressed on CD45+ ve T-cells, immature thymocytes, a subpopulation of peripheral B-cells and dendritic cells. CD150 is rapidly upregulated in T-cells, B-cells and dendritic cells after activation. In addition to the membrane form of CD150, activated T and B cells also express mRNA encoding a secreted form of CD150.
      Mouse anti Human CD150 antibody, clone A12 is reported to induce proliferation of pre-activated T cells (Cocks et al. 1995).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Cynomolgus monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Activated human peripheral blood mononuclear cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      interspecies interaction between organisms
      lymphocyte activation
      transmembrane receptor activity
      positive regulation of cell proliferation
      integral to membrane
      antigen binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD150 Antibody Formats

    Formats Clone Applications Sizes available
    CD150 Antibody : FITC A12 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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