CD15 Antibody | Bu28

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  • Mouse anti Human CD15
  • Mouse anti Human CD15
  • Mouse anti Human CD15:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Bu28
  • Isotype
    IgM
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2656FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2656F
    MCA2656Fdatasheet pdfdatasheet pdf0.2 mg
    MCA2656
    MCA2656TFdatasheet pdfdatasheet pdf25 µg
    MCA2656T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD15 antibody, clone Bu28 recognises human CD15, otherwise known as Lewis X and stage-specfic embryonic antigen-1 (SSEA-1). The CD15 antigen is itself a terminal trisaccharide, Gal-beta1-4 (Fuc-alpha1-3) GlcNAc (3-FAL), which is found on a variety of glycoproteins and glycolipids. CD15 is predominately expressed by peripheral blood granulocytes and various epithelial tissues. It plays an important role in cell adhesion and has been used as a marker for diagnosis of Hodgkin’s lymphoma.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Pack Size: 0.2 mgPurified IgM - liquid
      Pack Size: 25 µgPurified IgM
      Pack Size: 0.2 mgPurified IgM - liquid
      Pack Size: 25 µgPurified IgM
      Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
      Ig fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 1.0mg/ml
      Ig concentration 1.0mg/ml
      Ig concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD15 Antibody Formats

    Formats Clone Applications Sizes available
    CD15 Antibody : FITC Bu28 F 0.1 mg
    CD15 Antibody : Purified Bu28 F 25 µg | 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative ControlMCA692100 TestsF
        MCA692
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative ControlMCA692100 TestsF
        MCA692
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgM Negative Control:FITCMCA692F0.1 mgF
        MCA692F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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