CD143 Antibody | 9B9

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CD143 Antibody | 9B9 gallery image 1

Staining of CHO cells transfected with human CD143 (ACE) with Mouse anti Human CD143: FITC (MCA2057F)

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  • CD143 Antibody | 9B9 thumbnail image 1
  • Mouse anti Human CD143:FITC
  • Mouse anti Human CD143:FITC
  • Mouse anti Human CD143
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    9B9
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2057FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2057F
    MCA2057C *, E, F, IPdatasheet pdfdatasheet pdf1 ml
    MCA2057
    MCA2057FTFdatasheet pdfdatasheet pdf25 µg
    MCA2057FT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD143 antibody, clone 9B9 recognizes human somatic CD143, also know as angiotensin - converting enzyme (ACE). CD143 exists in two forms, a ~170 kDa somatic form and a ~90 kDa germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa.

      This antibody recognizes active ACE binding to an N-terminal domain epitope, different to that recognized by clone i2H5 (MCA2055 ).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Paraffin
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow CytometryNeat1/10
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Kidney
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD143 Antibody Formats

    Formats Clone Applications Sizes available
    CD143 Antibody : FITC 9B9 F 25 µg | 0.1 mg
    CD143 Antibody : S/N 9B9 C *, E, F, IP 1 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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          Recommended Positive Controls

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              References

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