CD143 Antibody | 2E2

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  • Mouse anti Human CD143
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2E2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2056E, P *, WBdatasheet pdfdatasheet pdf1 ml
    MCA2056
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD143 antibody, clone 2E2 recognizes human CD143, also know as angiotensin - converting enzyme (ACE). CD143 exists in two forms, a 170 kDa somatic form and a 90 kDa germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone recognises denatured somatic form of ACE, binding to an epitope in the C domain, and has been reported to be active in western blotting (Balyasnikova et al. 2003). Mouse anti Human CD143 antibody, clone 2E2 was produced in the same fusion as clone 3C5 (MCA2056) which recognizes both the somatic and germinal denatured forms of CD143.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/501/100
      Immunohistology - Paraffin(1)Neat1/20
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Kidney (lung in rat studies)
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD143 Antibody Formats

    Formats Clone Applications Sizes available
    CD143 Antibody : S/N 2E2 E, P *, WB 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Kidney (lung in rat studies)

              References

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