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  • Recombinant Human CD141
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP251FNdatasheet pdfdatasheet pdf10 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Recombinant Human CD141 antigen produced in HEK293 cells represents full length human CD141, otherwise known as Thrombomodulin, is a transmembrane glycoprotein specifically synthesized by endothelial cells, which plays a critical role in the activation/conversion of protein C into protein Ca, through the formation of a complex with the coagulation factor Thrombin. The subsequent cleavage and deactivation of the coagulation factors Va and factor VIIIa by protein Ca, acts to reduce Thrombin production and hence prevent further coagulation.

      Plasma levels of CD141 are increased in diabetes mellitus and SLE, and Thrombomodulin can correlate with the pathogenesis of the cardiovascular diseases thrombosis and atherosclerosis.

      Recombinant soluble Thrombomodulin contains 491 amino acid residues, including the extracellular domain of Thrombomodulin.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Further may be prepared in a buffer containing 0,1% BSA.
    • Preparation
      Purified recombinant CD141 expressed in HEK293 cells
    • Preservative Stabilisers
      None present
    • Activity
      Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of Thrombin. The specific activity is >500 pmoles/min/ug.
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
      10mM Sodium Phosphate
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      protein binding
      integral to plasma membrane
      negative regulation of platelet activation
      leukocyte migration
      calcium ion binding
      blood coagulation
      transmembrane receptor activity
      negative regulation of fibrinolysis
      cell surface
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD141 Formats

    Formats Applications Sizes available
    CD141 : Rec. Protein FN 10 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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