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CD140a Antibody | 35248 (57/20)

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  • Mouse anti Human CD140a

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  • Product Type
    Monoclonal Antibody
  • Clone
    35248 (57/20)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0100-0220E, P, WBdatasheet pdfdatasheet pdf0.1 mg
    0100-0220
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CD140a antibody, clone 35248 recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kDa single pass type I transmembrane glycoprotein.

      PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains (Hart et al. 1990). The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA (Hart et al. 1988). Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases (Westermark 1995).

      Mouse anti Human CD140a antibody, clone 35248 has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 µg/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5 µg/ml1 µg/ml
      Immunohistology - Paraffin10µg/ml20µg/ml
      Western Blotting1 µg/ml2 µg/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD140a Antibody Formats

    Formats Clone Applications Sizes available
    CD140a Antibody : Purified 35248 (57/20) E, P, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              1. De Paula, M.L. et al. (2014) The PTEN inhibitor bisperoxovanadium enhances myelination by amplifying IGF-1 signaling in rat and human oligodendrocyte progenitors.
              Glia. 62 (1): 64-77.
              2. Preisner, A. et al. (2015) Non-steroidal anti-inflammatory drug indometacin enhances endogenous remyelination.
              Acta Neuropathol. 130 (2): 247-61.
              3. Kuroda, M. et al. (2017) Peripherally derived FGF21 promotes remyelination in the central nervous system.
              J Clin Invest. 127 (9): 3496-509.

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