CD13 Antibody (PrecisionAbTM Antibody) | OTI2F10

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CD13 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI2F10 gallery image 1

Western blot analysis of whole cell lysates probed with CD13 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to CD13 (molecular weight 140-160 kDa)

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  • Mouse anti CD13
(Rated 0.0 out of 5 based on 0 customer reviews)
    Mouse anti human CD13 antibody recognizes aminopeptidase N, also known as ANPEP, AP-M, AP-N or alanyl aminopeptidase. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00367WBdatasheet pdfdatasheet pdf100 µl
      VMA00367
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human CD13 antibody recognizes CD13, also known as ANPEP, AP-M, AP-N, alanyl aminopeptidase, aminopeptidase M, aminopeptidase N, hAPN, microsomal aminopeptidase and myeloid plasma membrane glycoprotein CD13.

        Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical (provided by RefSeq, Jul 2008). The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in ANPEP appear to be a cause of various types of leukemia or lymphoma.

        Mouse anti Human CD13 antibody detects a band of 140-160 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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