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  • Recombinant Human CD120b
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP215E, FN, WBdatasheet pdfdatasheet pdf20 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant human CD120b is an 18.9 kDa peptide comprised of 174 amino acids representing the soluble form of CD120b. Soluble human CD120b, otherwise known as sTNF receptor type II (sTNFRII), is a member of the tumour necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE.

      CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFRII acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.2ml distilled water. For extended storage the addition of 0.1% BSA is recommended.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant CD120b expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.125 µg/ml.
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution.
    • Molecular Weight
      18.9kD (174 amino acid residues)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      membrane raft
      integral to membrane
      plasma membrane
      tumor necrosis factor receptor activity
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4 ng/well
      Functional Assays0.125ug/ml
      Western Blotting1.53.0 ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      Recombinant human CD120b may be used as a standard for ELISA applications with either a purified human CD120b antibody (AHP1833) or a biotinylated human CD120b antibody (AHP1833B).
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Recombinant human CD120b may be used as the positive control in Western Blotting applications with either a purified human CD120b antibody (AHP1833) or a biotinylated human CD120b antibody (AHP1833B).
    • Instructions For Use

    Additional CD120b Formats

    Formats Applications Sizes available
    CD120b : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Human CD120b:BiotinAHP1833B50 µgE, WB
          Rabbit anti Human CD120bAHP18330.1 mgE, FN, IF, WB

          Recommended Positive Controls

            Histology Controls

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