CD11b Antibody (PrecisionAbTM Antibody) | 2D11

CD11b Antibody (PrecisionAb<sup>TM</sup> Antibody) | 2D11 gallery image 1

Western blot analysis of whole cell lysates probed with CD11b antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 200 second exposure. Arrow points to CD11b (molecular weight 170 kDa)

  • CD11b Antibody (PrecisionAb<sup>TM</sup> Antibody) | 2D11 thumbnail image 1
  • Mouse anti CD11b
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    Mouse anti-human CD11b antibody recognizes integrin alpha-M, also known as CR-3 alpha chain, antigen CD11b (p170), cell surface glycoprotein MAC-1 subunit alpha, integrin alpha-M, leukocyte adhesion receptor MO1, macrophage antigen alpha polypeptide or neutrophil adherence receptor alpha-M subunit. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00481WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Mouse anti Human CD11b antibody recognizes CD11b also known as integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, cell surface glycoprotein MAC-1 subunit alpha, leukocyte adhesion receptor MO1, macrophage antigen alpha polypeptide or neutrophil adherence receptor alpha-M subunit.

        The ITGAM gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for ITGAM (provided by RefSeq, Mar 2009).

        Mouse anti Human CD11b antibody detects a band of 170 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
      • Western Blotting
        Anti CD11b detects a band of approximately 170 kDa in TF-1 cell lysate
      • Product Form
        Purified IgG - liquid
      • Preparation
        Purified IgG prepared by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length recombinant protein of human CD11b produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        integrin-mediated signaling pathway
        blood coagulation
        integrin complex
        receptor activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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