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CD102 Antibody | CBRIC2/2

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CD102 Antibody | CBRIC2/2 gallery image 1

Figure A. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse anti Human CD102 (MCA2290PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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CD102 Antibody | CBRIC2/2 gallery image 2

Figure A. Alexa Fluor 700 conjugated mouse anti human CD3 (MCA463A700) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 700 conjugated mouse anti human CD3 (MCA463A700) and FITC conjugated mouse anti human CD102 (MCA2290F). All experiments performed on red cell lysed human blood gated on lymphocytes in the presence of human seroblock (BUF070A). Data acquired on the ZE5™ Cell Analyzer.

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  • CD102 Antibody | CBRIC2/2 thumbnail image 1
  • CD102 Antibody | CBRIC2/2 thumbnail image 2
  • Mouse anti Human CD102
  • Mouse anti Human CD102:Alexa Fluor® 647
  • Mouse anti Human CD102:FITC
  • Mouse anti Human CD102:Alexa Fluor® 647

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  • Product Type
    Monoclonal Antibody
  • Clone
    CBRIC2/2
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2290FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2290F
    MCA2290C, F, IPdatasheet pdfdatasheet pdf0.2 mg
    MCA2290
    MCA2290A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2290A647
    MCA2290A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2290A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD102 antibody, clone CBRIC2/2 recognizes human CD102, also known as intercellular adhesion molecule-2 (ICAM-2). CD102 is a glycosylated cell surface protein that is broadly expressed on most leucocytes and is strongly expressed by endothelial cells. CD102 interacts with the CD11a/CD18 (LFA-1). Reports suggest that the CD102 may play a role in lymphocyte re-circulation and T-cell activation.

      Mouse anti Human CD102 antibody, clone CBRIC2/2 is reported to inhibit interactions between CD102 and LFA-1 (De Fougerolles et al. 1991).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647- liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Alexa Fluor® 647- liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Transfected COS cells expressing ICAM-2 cDNA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Cells from immunised BALB/c mice were fused with cells of the Murine P3X63Ag.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      GO:0050776
      regulation of immune response
      Related reagents
      GO:0005178
      integrin binding
      Related reagents
      GO:0005887
      integral to plasma membrane
      Related reagents
      GO:0016337
      cell-cell adhesion
      Related reagents
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD102 Antibody Formats

    Formats Clone Applications Sizes available
    CD102 Antibody : Alexa Fluor® 647 CBRIC2/2 F 25 Tests/0.25ml | 100 Tests/1ml
    CD102 Antibody : FITC CBRIC2/2 F 0.1 mg
    CD102 Antibody : Purified CBRIC2/2 C, F, IP 0.2 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              Source Reference

              1. de Fougerolles, A.R. et al. (1991) Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
              J Exp Med. 174 (1): 253-67.

              References

              1. Xu, H. et al. (1996) Characterization of murine intercellular adhesion molecule-2.
              J Immunol. 156 (12): 4909-14.
              2. Diacovo, T.G. et al. (1994) A functional integrin ligand on the surface of platelets: intercellular adhesion molecule-2.
              J Clin Invest. 94 (3): 1243-51.
              3. Porter, J.C. & Hall, A. (2009) Epithelial ICAM-1 and ICAM-2 regulate the egression of human T cells across the bronchial epithelium.
              FASEB J. 23 (2): 492-502.
              4. Banerjee, P. et al. (2007) Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells.
              J Virol. 81: 9707-17.
              5. Arjmandi A et al. (2009) Dendritic cell adhesion to cerebral endothelium: role of endothelial cell adhesion molecules and their ligands.
              J Neuropathol Exp Neurol. 68 (3): 300-13.

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