CCT Beta Antibody (PrecisionAbTM Antibody)

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CCT Beta Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with CCT beta antibody followed by detection with HRP conjugated Goat anti Rat IgG (1/10,000, STAR72) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to CCT beta (molecular weight 57 kDa)

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  • Rat anti CCT Beta
  • CCT Beta Antibody with Control Lysate
  • CCT Beta Antibody with Control Lysate
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    CCT beta antibody recognizes T-complex protein 1 subunit beta, also known as TCP-1-beta, CCT2 or CCT beta. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2b
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00026WBdatasheet pdfdatasheet pdf100 µl
      VMA00026
      VMA00026KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00026K
      VMA00026KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00026KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rat anti Human CCT beta antibody recognizes human T-complex protein 1 subunit beta, also known as CCT beta, CCT2 or TCP-1-beta. CCT beta is a 535 amino acid cytoplasmically expressed chaperonin molecule involved in protein folding and has been shown to be involved in actin interaction (Llorca et al. 1999). Two isoforms of CCT beta have been described, arising from alternative splicing. It is not clear whether this Rat anti Human CCT beta antibody recognizes the shorter isoform 2, lacking the N-terminal 47 amino acids seen in isoform 1.

        Rat anti Human CCT beta antibody recognizes CCT beta as a single band of ~ 57 kDa in multiple human cell line lysates by western blotting under reducing conditions and is cross reactive with both mouse and rat cell line lysates.
      • Target Species
        Human
      • Western Blotting
        Anti CCT beta antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
        Anti CCT beta antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
        Anti CCT beta antibody detects a band of approximately 57 kDa in Jurkat cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Ratyes
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 10 Westerns100μl Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 2 Westerns20μl Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 10 Westerns100μl Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
        Pack Size: 2 Westerns20μl Rat monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Mouse CCT beta peptide conjugated to purified tuberculin protein derivative
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        ATP binding
        'de novo' posttranslational protein folding
        nucleus
        unfolded protein binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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