CBX1 Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-cbx1-antibody-vpa00609.html?RenderPageSpecific=1
CBX1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with CBX1 antibody followed by detection with HRP conjugated Goat anti Rabbit IgG (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 15 second exposure. Arrow points to CBX1 (molecular weight 25 kDa)

Enlarge
  • CBX1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • CBX1 Antibody with Control Lysate
  • Rabbit anti CBX1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    PrecisionAb™ Polyclonal
  • Isotype
    Polyclonal IgG
  • Format
    Purified
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    VPA00609WBdatasheet pdfdatasheet pdf100 µl
    VPA00609
    VPA00609KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
    VPA00609KT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    References
    Reviews
    -
    • Rabbit anti Human CBX1 antibody recognizes the chromobox protein homolog 1, also known as HP1 beta homolog, heterochromatin protein 1 homolog beta, heterochromatin protein 1-beta, heterochromatin protein p25 beta or modifier 1 protein.

      The CBX1 gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified (provided by RefSeq, Jul 2008).

      Rabbit anti Human CBX1 antibody detects a band of 25 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Target Species
      Human
    • Western Blotting
      Rabbit anti CBX1 detects a band of approximately 25 kDa in Jurkat cell lysates
      Rabbit anti CBX1 detects a band of approximately 25 kDa in Jurkat cell lysates
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Preparation
      20μl Rabbit polyclonal antibody purified by affinity chromatography
      Rabbit polyclonal antibody purified by affinity chromatography
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Preservative Stabilisers
      0.09% Sodium Azide
      2% Sucrose
      0.09% Sodium Azide
      2% Sucrose
    • Immunogen
      Synthetic peptide encompassing part of the N terminal region of human CBX1
    • Instructions for Use
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
    • Lysate Composition
      400μg Jurkat lysate lyophilized in RIPA buffer.
    • Lysate Reconstitution
      - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
      - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
      Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
    • Storage
      Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

      Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
    • Shelf Life
      As supplied, 12 months from date of despatch
      As supplied, 12 months from date of despatch.
    • Acknowledgements
      PrecisionAb™ is a trademark of Bio-Rad Laboratories
      PrecisionAb™ is a trademark of Bio-Rad Laboratories.
    • Regulatory
      For research purposes only
    • GO Terms
      enzyme binding
      nuclear heterochromatin
      chromatin binding
      nucleoplasm
      spindle
      chromosome, centromeric region
      chromatin assembly or disassembly
    • UniProt
    • Entrez Gene
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
      STAR208P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Write your review

              Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

              Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

              Contact Information


              Online Payment Methods:

              1. Invoice / Purchase Order
              2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
              credit cards accepted
              • View more products with "CBX1" specificity.