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Caveolin 1 Antibody
Western blot analysis of Human Spleen lysate (35µg protein in RIPA buffer) using Goat anti caveolin-1 antibody (AHP1058) at 0.01μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Enlarge- Goat anti Caveolin 1
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Product TypePolyclonal Antibody
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IsotypePolyclonal IgG
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
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Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Goat anti Human Caveolin 1 antibody recognizes human caveolin-1, a 178 amino acid, ~21kDa membrane associated protein. Caveolin 1 is a scaffolding protein and the main component of the caveolae plasma membranes found in most cell types (Anderson 1998). Two isoforms of caveolin are generated by alternative initiation, they differ in their N-terminal region with caveolin 1β lacking amino acids 1-31 present in caveolin 1α (Vainonen et al. 2004). Goat anti Human caveolin 1 antibody recognizes epitopes within the region between amino acids 34-41 of caveolin 1α and is therefore expected to recognize both isoforms of caveolin 1
Caveolin1 is highly expressed in terminally differentiated or quiescent cells, suggesting a possible role for caveolin 1 as a negative regulator of cell proliferation (Galbiati et al.2001).
Caveolin1 has been implicated in the pathogenesis of oncogenic cell transformation, tumorigenesis, and metastasis (Núñez-Wehinger et al. 2014).
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Intended Use
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Target SpeciesHuman
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Species Cross-Reactivity
N.B. Antibody reactivity and working conditions may vary between species.Target Species Cross Reactivity Rat Expected from Sequence Mouse Expected from Sequence Dog Expected from Sequence Pig Expected from Sequence -
Product FormPurified IgG - liquid
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Reconstitution
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Preparation
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Antiserum PreparationAntisera to Caveolin 1 were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
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Preservative Stabilisers
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin -
ImmunogenPeptide sequence C-DELSEKQVYDAH from internal region of Caveolin 1.
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Purity
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Approx. Protein ConcentrationsIgG concentration 0.5mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionTRIS buffered saline
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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GO Termsreceptor bindingnitric-oxide synthase bindingnegative regulation of gene-specific transcription from RNA polymerase II promoterlipid particlepositive regulation of peptidyl-serine phosphorylationnegative regulation of canonical Wnt receptor signaling pathwayvesicle organizationcellular response to starvationnegative regulation of endothelial cell proliferationblood coagulationcaveola assemblyprotein complex scaffoldmammary gland involutionreceptor internalizationnitric oxide homeostasiscalcium ion transportendoplasmic reticulumnegative regulation of protein bindingnegative regulation of MAPKKK cascadeinactivation of MAPK activityT cell costimulationcaveolacholesterol homeostasisGolgi membraneregulation of blood coagulationapical plasma membranevasculogenesistriglyceride metabolic processprotein bindingpositive regulation of calcium ion transport into cytosolpositive regulation of vasoconstrictionresponse to hypoxianegative regulation of nitric oxide biosynthetic processresponse to calcium ionperinuclear region of cytoplasmregulation of nitric-oxide synthase activitymaintenance of protein location in cellskeletal muscle tissue developmentprotein homooligomerizationpeptidase activator activityinterspecies interaction between organismsnegative regulation of peptidyl-serine phosphorylationpositive regulation of canonical Wnt receptor signaling pathwaynitric oxide metabolic processnegative regulation of BMP signaling pathwayleukocyte migrationcytosolic calcium ion homeostasislipid storageresponse to estrogen stimulusregulation of smooth muscle contractioncholesterol bindingnegative regulation of epithelial cell differentiationbasolateral plasma membranemembrane depolarizationregulation of fatty acid metabolic processresponse to progesterone stimuluspositive regulation of metalloenzyme activity
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA 1/32,000 Western Blotting 0.3 1.0ug/ml - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western BlottingAHP1058 detects a band of approximately 21Da and 25kDa in human heart, lung and spleen lysates.
- Instructions For Use
Additional Caveolin 1 Antibody Formats
| Formats | Applications | Sizes available |
|---|---|---|
| Caveolin 1 Antibody : Purified | E, WB | 0.1 mg |
- Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Goat IgG (Fc):FITC | STAR122F | 1 mg | F | STAR122F | |
| Rabbit anti Goat IgG (Fc):HRP | STAR122P | 1 mg | C, E, WB | STAR122P |
Recommended Negative Isotype Control
Useful Reagents
Recommended Positive Controls
Histology Controls
Application Name Reference Images Western Blotting
References
1. Hommel, A. et al. (2010) The ARF-like GTPase ARFRP1 is essential for lipid droplet growth and is involved in the regulation of lipolysis.Mol Cell Biol. 30: 1231-42.
Further Reading
1. Williams, T.M. & Lisanti, M.P. (2005) Caveolin-1 in oncogenic transformation, cancer, and metastasis.Am J Physiol Cell Physiol. 288 (3): C494-506.
2. Liu, P. et al. (2002) Multiple functions of caveolin-1.J Biol Chem. 277 (44): 41295-8.
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Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
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