Cathepsin K Antibody | 2F1

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Cathepsin K Antibody | 2F1 gallery image 1

Staining of paraffin-embedded human kidney with Mouse anti Human cathepsin K (MCA5232Z)

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  • Cathepsin K Antibody | 2F1 thumbnail image 1
  • Mouse anti Human Cathepsin K:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    2F1
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5232ZPdatasheet pdfdatasheet pdf0.1 mg
    MCA5232Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human cathepsin K antibody, clone 2F1 recognizes human cathepsin K, also known as cathepsin O, cathepsin O2 or cathepsin X. Cathepsin K is a 215 amino acid ~40 kDa molecule (having an additional 114 amino acid propeptide and signal peptide sequence) with broad proteolytic activity. Cathepsin K is involved in osteoclastic bone resorption and plays roles in extracellular matrix degradation (Inaoka et al. 1995). Cathepsin K also plays a role in polmonary cavitation and collagen turnover in patients with pulmonary tuberculosis (Kubler et al. 2015).

      Mouse anti Human cathepsin K antibody, clone 2F1 has been successfully used for the detection of cathepsin K in rabbit tissues by immunohistochemistry on formalin fixed, paraffin embedded material following antigen retrieval (Kubler et al. 2015).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 220 - 331 of human CATHEPSIN K
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      proteolysis
      protein binding
      lysosome
      cysteine-type endopeptidase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Cathepsin K Antibody Formats

    Formats Clone Applications Sizes available
    Cathepsin K Antibody : Preservative Free 2F1 P 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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