Catenin Delta 1 Antibody (PrecisionAbTM Antibody)

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Catenin Delta 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with catenin delta 1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 180 second exposure. Arrow points to catenin delta 1 (molecular weight 100 kDa)

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  • Catenin Delta 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Catenin Delta 1 Antibody with Control Lysate
  • Catenin Delta 1 Antibody with Control Lysate
  • Rabbit anti Catenin Delta 1
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    Rabbit anti-human CTNND1 antibody recognizes catenin delta-1, also known as cadherin-associated Src substrate or p120 catenin. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00262WBdatasheet pdfdatasheet pdf100 µl
      VPA00262
      VPA00262KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00262K
      VPA00262KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00262KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human catenin delta 1 antibody recognizes catenin delta-1, also known as CTNND1, CAS, CTNND, cadherin-associated Src substrate, p120 and p120 catenin.

        Catenin delta 1 is a member of the Armadillo protein family. This family of proteins functions in adhesion between cells and signal transduction. Multiple translation initiation codons and alternative splicing result in many different isoforms being translated, some with undetermined function. Read-through transcription also exists between catenin delta 1 and the neighboring upstream thioredoxin-related transmembrane protein 2 (TMX2) gene (provided by RefSeq, Dec 2010).

        Rabbit anti Human catenin delta-1 antibody detects a band of 100 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti catenin delta 1 detects a band of approximately 100 kDa in A431 cell lysate
        Anti catenin delta 1 detects a band of approximately 100 kDa in A431 cell lysate
        Anti catenin delta 1 detects a band of approximately 100 kDa in A431 cell lysate
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        Pack Size: 10 Westerns0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        Pack Size: 2 Westerns0.09% sodium azide (NaN3), 1% bovine serum albumin.
        Pack Size: 10 Westerns0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        Pack Size: 2 Westerns0.09% sodium azide (NaN3), 1% bovine serum albumin.
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
      • Immunogen
        KLH-conjugated synthetic peptide corresponding to aa 160-200 of human catenin delta 1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A431 lysate lyophilized in RIPA buffer
        400μg A431 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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