Caspase-9 p10 Antibody

//www.bio-rad-antibodies.com/human-caspase-9-p10-antibody-ahp2419.html
Caspase-9 p10 Antibody gallery image 1

Western blot analysis of (A) HeLa and (B) Jurkat whole cell lysates probed with Rabbit anti caspase-9 p10 antibody at a dilution of 1/200

Enlarge
  • Caspase-9 p10 Antibody thumbnail image 1
  • Rabbit anti Caspase-9 p10
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2419WBdatasheet pdfdatasheet pdf0.1 mg
    AHP2419
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti caspase-9 p10 antibody recognizes caspase-9, a member of the cysteine-aspartic acid protease family. The active form of caspase-9 is generated by cleavage of the pro-caspase-9 protein into fragments, known as the large and small fragments. The trigger for caspase-9 cleavage is the formation of the apoptosome (comprising of cytochrome c, APAF-1, dATP and pro-caspase-9), upon stimulation of the intrinsic apoptosis pathway.

      The rabbit anti caspase-9 antibody was raised against an internal peptide that is present in one of the small fragments (p10) generated in the pro-caspase-9 cleavage process at Asp330 (Zou et al. 2002). The antibody will therefore detect pro-caspase-9 (as the motif is present) and the p10 caspase-9 fragment. The antibody can therefore be used to distinguish the p10 caspase-9 fragment from other pro-caspase-9 cleavage products.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Antiserum Preparation
      Antiserum to caspase-9 p10 was raised by repeated immunisation of rabbits with highly purified antigen
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.2% Gelatin
    • Immunogen
      Peptide mapping to an internal region of human caspase-9 p10 subunit
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      nerve growth factor receptor signaling pathway
      protein binding
      activation of caspase activity by cytochrome c
      phosphatidylinositol-mediated signaling
      induction of apoptosis by intracellular signals
      cysteine-type endopeptidase activity
      cytosol
      proteolysis
      enzyme activator activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/200

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti caspase-9 p10 recognizes a band of approximately 47 kDa when run under reducing conditions without induction of apoptosis
    • Instructions For Use

    Additional Caspase-9 p10 Antibody Formats

    Formats Applications Sizes available
    Caspase-9 p10 Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...

              You May Also Be Interested In...