Caspase-7 (Active) Antibody

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Western blot analysis of Jurkat cell lysates, from cells treated with camptothecin for 6 hours, probed with Rabbit anti caspase-7 (active) antibody

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  • Rabbit anti Caspase-7 (Active)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2287IP, P, WBdatasheet pdfdatasheet pdf50 µg
    AHP2287
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti caspase-7 antibody recognizes caspase-7. Caspase-7 is a member of the cysteine-aspartic acid protease family. The active form of caspase-7 is generated via cleavage of the pro-caspase-7 by caspase 3, 9 or 10. Caspase-7 is an effector caspase that plays a role in the cleavage of the DNA repair and apoptosis mediating PARP (Poly ADP-ribose polymerase) proteins.Caspase activity can be inhibited by c-IAP1, c-IAP2, XIAP and other members of the inhibitor of apoptosis protein family.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      50% Glycerol
      1% Bovine serum Albumin
      0.02% Thiomersal
    • Immunogen
      Synthetic peptide surrounding amino acid 195 of human caspase-7
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      proteolysis
      protein binding
      cysteine-type endopeptidase activity
      induction of apoptosis by intracellular signals
      endoplasmic reticulum membrane
      cytosol
      mitochondrial membrane
      cellular component disassembly involved in apoptosis
      activation of caspase activity by cytochrome c
      nucleoplasm
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1020 ug/ml
      Immunoprecipitation1020 ug/ml
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti caspase-7 recognizes the large subunit of cleaved caspase-7 (approximately 20 kDa) in lysate from Jurkat cells treated with 2 mM camptothecin for 6hrs
    • Instructions For Use

    Additional Caspase-7 (Active) Antibody Formats

    Formats Applications Sizes available
    Caspase-7 (Active) Antibody : Purified IP, P, WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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