Caspase-3 Antibody

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Caspase-3 Antibody gallery image 1

Published customer image:
Rabbit anti Human Caspase-3 antibody used foir the detection of caspase-3 expressing cells in Salmonella enterica infected mouse liver vibratome sections by immunohistochemistry.
Image caption:
Infected Tissues Have Significant Inflammatory Lesions at 1 wk and 3 wk but Contain Few Caspase-3-Positive Cells. Light microscopy of hematoxylin (purple), eosin (pink), and caspase-3 (brown, arrows) stained liver thin sections over an infection time course. Enlarged images of the boxed regions on the left (200-µm scale bars) are shown on the right (50-µm scale bars). Arrowheads show inflammatory lesions: (A–B) 4 d post-infection; (C–D) 1 wk; (E–F) 3 wk; and (G–H) 8 wk.

From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.
PLoS Pathog 3(12): e193.

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  • Caspase-3 Antibody thumbnail image 1
  • Rabbit anti Human Caspase-3
  • Rabbit anti Human Caspase-3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP476P *, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP476
    AHP476TP *datasheet pdfdatasheet pdf10 µl
    AHP476T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human Caspase-3 antibody recognizes the Caspase-3 cell death protease, also known as CPP32, YAMA and apopain. Caspase-3 is a ~32 kDa protein, activated during apoptosis by cleavage into 17 kDa and 11 kDa fragments. Rabbit anti Human Caspase-3 antibody recognizes both intact Caspase-3 and the 17 kDa fragment, but not the 11 kDa fragment.

      In immunohistochemical studies Caspase-3 expression has been shown to be widespread, but not present in all cell types. Differences in the level of Caspase-3 have been reported in cells of short-lived nature (e.g. germinal centre B cells) and those that are long lived (e.g. mantle zone B cells).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
      Immunohistology - Paraffin(1)1/1001/500
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin section
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000
      Immunohistology - Paraffin(1)1/1001/500
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin section

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Lymph node/kidney
    • Histology Positive Control Tissue
      Lymph node/kidney
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Caspase-3 Antibody Formats

    Formats Applications Sizes available
    Caspase-3 Antibody : Serum P *, WB 0.1 ml | 10 µl
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
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      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Lymph node/kidney
              Lymph node/kidney
              • Application NameReference Images
                Western Blotting

              References

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