Caspase-3 (Active) Antibody

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Western blot analysis of Jurkat cell lysates, from cells treated with camptothecin, probed with Rabbit anti caspase-3 (active) antibody

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  • Rabbit anti Caspase-3 (Active)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2286P, WBdatasheet pdfdatasheet pdf50 µg
    AHP2286
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti caspase-3 antibody recognizes caspase-3 also known as cysteine protease CPP32. Caspase-3 as the name implies is a member of the cysteine-aspartic acid protease family. Caspase-3 gets cleaved from an inactive 32 kDa zymogen into p17 and p12 chains. These two subunits dimerize to form the active caspase 3 protein, which acts as an effector caspase that cleaves and activates caspase-6, 7 and 9. Via this process caspase-3 plays an important role in mediating apoptosis.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin2040 ug/ml
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti caspase -3 recognizes the p17 fragment of the active caspase-3 (approximately 17 kDa) in lysate from Jurkat cells treated with 2 µM camptothecin
    • Instructions For Use

    Additional Caspase-3 (Active) Antibody Formats

    Formats Applications Sizes available
    Caspase-3 (Active) Antibody : Purified P, WB 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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