Caspase-1 Antibody | 3D2

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Staining of paraffin embedded human hepatocellular carcinoma with Mouse anti Human CASPASE-1:Azide Free (MCA2979Z)

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  • Mouse anti Human Caspase-1:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    3D2
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2979ZP *datasheet pdfdatasheet pdf0.1 mg
    MCA2979Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti ~Human Caspase-1 antibody, clone 3D2 recognizes human caspase-1, also known as Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, ICE or p45. Caspase-1 is a 404 amino acid ~45 kDa cytoplasmic thiol protease that cleaves IL-1 beta, releasing the mature cytokine. Caspase-1 is expressed in multiple organs with highest levels in spleen and lung.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1-100 of human CASP1
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      signal transduction
      proteolysis
      caspase activator activity
      apoptosis
      cytosol
      cysteine-type endopeptidase activity
      protein binding
      positive regulation of I-kappaB kinase/NF-kappaB cascade
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Caspase-1 Antibody Formats

    Formats Clone Applications Sizes available
    Caspase-1 Antibody : Preservative Free 3D2 P * 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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