Casein Kinase 2 Antibody | AbD05928

Casein Kinase 2 Antibody | AbD05928 gallery image 1

Casein kinase 2 alpha 1 detected in A375 human malignant melanoma cell lysates with Human anti casein kinase 2 alpha 1 (HCA064)

Casein Kinase 2 Antibody | AbD05928 gallery image 2

Western blot analysis of Jurkat human T cell leukemia whole cell lysate probed with Human anti Human casein kinase 2 alpha 1 antibody (HCA064) followed by followed by HRP conjugated F(ab')2 Goat anti Human IgG, visualized by chemiluminescence

  • Casein Kinase 2 Antibody | AbD05928 thumbnail image 1
  • Casein Kinase 2 Antibody | AbD05928 thumbnail image 2
  • Human anti Casein Kinase 2 Alpha 1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab monovalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA064E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Human anti casein kinase 2 alpha 1 antibody, clone AbD05928 recognizes human casein kinase 2 alpha 1. Casein kinase II (CK2) is a heterotetrameric serine/threonine protein kinase consisting of two 44 kDa catalytic subunits (CK2-alpha) and two 26 kDa regulatory subunits (CK2-beta). The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. CK2 is constitutively expressed in most cells and tissues and is highly conserved among eukaryotes. It participates in a wide variety of cellular processes including protein kinase networks that control cell cycle progression and cellular stress responses with over 300 substrates. Consequently, fluctuations in the expression of CK II have been linked with various human diseases and most notably, certain cancers (breast, prostate, lung etc).
    • Intended Use
    • Target Species
    • Product Form
      A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.01% Thiomersal
    • Immunogen
      Full length recombinant human CK2-alpha1 expressed in Sf9 cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      ATP binding
      Wnt receptor signaling pathway
      protein serine/threonine kinase activity
      NuRD complex
      plasma membrane
      axon guidance
      protein N-terminus binding
      Sin3 complex
      protein phosphorylation
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
      His-tag is a registered trademark of EMD Biosciences.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      HCA064 detects a band of approximately 44 kDa in HCT-116 cell lysates
    • Instructions For Use

    Additional Casein Kinase 2 Antibody Formats

    Formats Clone Applications Sizes available
    Casein Kinase 2 Antibody : Purified AbD05928 E, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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