Carbonic Anhydrase Ix Antibody (PrecisionAbTM Antibody)

Carbonic Anhydrase Ix Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with carbonic anhydrase IX antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to carbonic anhydrase IX (molecular weight 35-58 kDa)

  • Carbonic Anhydrase Ix Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Rabbit anti Carbonic Anhydrase Ix
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    Carbonic anhydrase IX antibody recognizes carbonic anhydrase IX, also know as CA9 or carbonate dehydratase IX.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00813WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Rabbit anti Human carbonic anhydrase IX antibody recognizes carbonic anhydrase IX, also know as carbonic anhydrase 9 (CA9) or carbonate dehydratase IX.

        Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12 (provided by RefSeq, Jun 2014).

        Rabbit anti Human carbonic anhydrase IX antibody detects bands of 35-58 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
      • Western Blotting
        Anti carbonic anhydrase IX antibody recognizes bands of approximately 35-58 kDa in HT-29 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Rabbit polyclonal antibody purified by affinity chromatography on immunogen
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide
        <50% Glycerol
      • Immunogen
        Recombinant protein of human carbonic anhydrase IX
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        carbonate dehydratase activity
        one-carbon metabolic process
        microvillus membrane
        zinc ion binding
        integral to membrane
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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