Carbonic Anhydrase II Antibody (PrecisionAbTM Antibody)

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Carbonic Anhydrase II Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with carbonic anhydrase II antibody followed by detection with HRP conjugated Rabbit anti Sheep IgG (H/L) (1/10,000, 5184-2504) and visualized on the ChemiDoc MP with 100 second exposure. Arrow points to carbonic anhydrase II (molecular weight 29 kDa)

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  • Carbonic Anhydrase II Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Carbonic Anhydrase II Antibody with Control Lysate
  • Carbonic Anhydrase II Antibody with Control Lysate
  • Sheep anti Carbonic Anhydrase II
(Rated 0.0 out of 5 based on 0 customer reviews)
    Carbonic anhydrase II antibody recognizes carbonic anhydrase 2, also known as carbonate dehydratase II, carbonic anhydrase C, CAC, carbonic anhydrase II or CA-II. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00073WBdatasheet pdfdatasheet pdf100 µl
      VPA00073
      VPA00073KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00073K
      VPA00073KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00073KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Sheep anti Human carbonic anhydrase II antibody recognizes carbonic anhydrase II (CA-II), also known as carbonate dehydratase II and carbonic anhydrase C (CAC). CA-II is a ubiquitous zinc enzyme that catalyzes the reversible conversion of carbonic acid (H2CO3) to carbon dioxide (CO2) and water (H2O). Carbonic anhydrase inhibitors have now been a mainstay of human clinical intervention for several decades, with at least 25 clinically used drugs that are CA inhibitors

        Sheep anti Human carbonic anhydrase II antibody detects a single band of 29 kDa in the human epidermoid carcinoma A431 cell line lysate. Cross reactivity with mouse cell lines is also seen.
      • Target Species
        Human
      • Western Blotting
        Sheep anti carbonic anhydrase II detects a band of approximately 29 kDa in A431 cell lysates.
        Sheep anti carbonic anhydrase II detects a band of approximately 29 kDa in A431 cell lysates.
        Sheep anti carbonic anhydrase II detects a band of approximately 29 kDa in A431 cell lysates.
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Sheep polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Sheep polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Sheep polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Sheep polyclonal antibody purified by affinity chromatography
        Sheep polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg A431 lysate lyophilized in RIPA buffer.
        400μg A431 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        apical part of cell
        carbonate dehydratase activity
        one-carbon metabolic process
        zinc ion binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Sheep IgG (H/L):HRP5184-25042 mgC, E, WB
        5184-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Sheep IgG (H/L):HRP5184-25042 mgC, E, WB
        5184-2504
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rabbit anti Sheep IgG (H/L):HRP5184-25042 mgC, E, WB
        5184-2504

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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