CAPZB Antibody

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CAPZB Antibody gallery image 1

Western blot analysis of CAPZB expression in cell lines of human and mouse origin, lane A Jurkat cell lysate, lane B HeLa lysate, lane C NIH-3T3 lysate probed with Goat anti Human CAPZB antibody (AHP707) at 0.01μgml-1

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CAPZB Antibody gallery image 2

Western blot analysis of CAPZB expression in heart lysates from (A) Mouse, (B) Rat, (C) Pig. Blots probed with Goat anti Human CAPZB antibody (AHP707) used at 0.1μgml-1

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  • Goat anti Human CAPZB
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP707WBdatasheet pdfdatasheet pdf0.1 mg
    AHP707
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human CAPZB antibody recognizes human F-actin capping protein muscle Z-line beta also known as CapZ beta or CAPZB. CAPZB is a member of the F-actin capping protein family involved in the growth regulation of actin filaments by binding to the barbed ends of fast growing actin filaments (Bai et al. 2011).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      BovineExpected from Sequence
      Ratyes
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to CAPZB were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Synthetic peptide NDLVEALKRKQQC corresponding to the C-terminus of CAPZB (NP_004921).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      F-actin capping protein complex
      actin binding
      actin filament capping
      cellular component movement
      WASH complex
      cytosol
      actin cytoskeleton organization
      blood coagulation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.5ug/ml2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP707 detects a band of approximately 28-30kDa in human liver extracts. A minimum incubation time of 1 hour is recommended for this antibody.
    • Instructions For Use

    Additional CAPZB Antibody Formats

    Formats Applications Sizes available
    CAPZB Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

              Further Reading

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