CaMKK Beta Antibody | 1A11

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CaMKK Beta Antibody | 1A11 gallery image 1

Western blot analysis of CaMKK Beta expression in IMR-32 using Mouse anti Human CaMKK Beta:Azide Free (MCA3720Z)

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  • Mouse anti Human CaMKK Beta:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    1A11
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3720ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA3720Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human CaMKK beta antibody, clone 1A11 recognizes human Calcium/calmodulin-dependent protein kinase kinase 2, also known as CaMKK Beta, Calcium/calmodulin-dependent protein kinase kinase beta or CaMKK 2. CaMKK Beta is a 587 amino acid ~70 kDa cytoplasmic enzyme involved in the calcium-triggered signaling cascade.
      Multiple isoforms of CaMKK beta with distinct kinase activities may be generated by alternative splicing (Hsu et al.2001) differing largely in regions surrounding the calmodulin binding domain at the caroboxy region of CaMKK beta. Mouse anti Human CaMKK beta antibody, clone 1A11, generated from an immunogen corresponding to the N-terminal region of CaMKK beta is expected to recognize all described isoforms of human Calcium/calmodulin-dependent protein kinase kinase 2.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 1 - 130 of human CaMKK Beta.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CaMKK Beta Antibody Formats

    Formats Clone Applications Sizes available
    CaMKK Beta Antibody : Preservative Free 1A11 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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