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Calcitonin Receptor Antibody | 30/7-9B4

Calcitonin Receptor Antibody | 30/7-9B4 gallery image 1

Published customer image:
Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 used for the identification of calcitonin receptor expressing cells in bovine cartilage explants by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunolocalization of the calcitonin receptor (CTR) in cartilage explants induced with or without T3 for 4 days. First panel shows the superficial layer of the cartilage, second panel the mid layer of the cartilage and the third panel the deep layer of the cartilage. Treatment scheme is indicated at the top of the pictures. The pictures are representative of 3 replicates from the same experiment. Positive staining is visualized by brown color.

From: Chen-An P, Andreassen KV, Henriksen K, Li Y, Karsdal MA, Bay-Jensen A-C (2012)
The Inhibitory Effect of Salmon Calcitonin on Tri-Iodothyronine Induction of Early Hypertrophy in Articular Cartilage.
PLoS ONE 7(6): e40081.

  • Calcitonin Receptor Antibody | 30/7-9B4 thumbnail image 1
  • Mouse anti Human Calcitonin Receptor
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5749E, F, IF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 recognizes the human calcitonin receptor, a 508 amino acid multi pass transmembrane membrane glycoprotein with seven transmembrane domains, coupled to G protein messenger systems. The calcitonin receptor is expressed in kidney, central and peripheral nervous systems and on osteoclasts. It is also expressed in a number of tissues during the development of the embryo and may be important in foetal morphogenesis.Clone MAb 9B4 recognises an epitope within the extra-cellular domain, the epitope is common to both C1a and C1b human isoforms.

      Calcitonin receptor expression is elevated in the inflammatory cells associated with diseased arteries and atherosclerotic plaques (Wookey et al. 2009) expression has also been noted within malignant cells of glioblastoma multiforme (Wookey et al. 2012).Several different isoforms of the calcitonin receptor, a result of alternative splicing and promotor usage have been identified. Mouse anti Human Calcitonin Receptor antibody, clone 30/7-9B4 was raised against the canonical isoform 1 but has been shown to bind to a truncated isoform lacking the N-terminal region of the receptor (Qi et al. 2012). The calcitonin receptor can associate with RAMP1, 2 and 3 to form discrete receptors for amylin

      Mouse anti human calcitonin receptor antibody, clone 30/7-9B4 has been successfully used for the demonstrating calcitonin receptor on human cryostat tissue section by immunohistochemistry and western blotting (Wookey et al. 2012).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G-Sepharose/dialysed against PBS from tissue culture supernatant.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide derived from sequence situated on the extra-cellular domain of human calcitonin receptor.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      positive regulation of adenylate cyclase activity
      elevation of cytosolic calcium ion concentration
      protein binding
      calcitonin receptor activity
      activation of adenylate cyclase activity by G-protein signaling pathway
      integral to plasma membrane
      response to glucocorticoid stimulus
      calcitonin binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Western Blotting
      Immunohistology - Paraffin(1)
      PLP fixation is recommended for optimal results.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human kidney
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Calcitonin Receptor Antibody Formats

    Formats Clone Applications Sizes available
    Calcitonin Receptor Antibody : Purified 30/7-9B4 E, F, IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000B150 TestsC, P

          Recommended Positive Controls

            Histology Controls

              Human kidney
                • Product Images

                • Calcitonin Receptor Antibody | 30/7-9B4 thumbnail image 1


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