Native Human Complement Component C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis.
Purified C1q from human serum - lyophilised
Reconstitute with 1.0ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. The solution may appear cloudy following reconstitution
SDS PAGE: >96% Single band at 410kDa in PAGE.
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml after reconstitution
Reagents In The Kit
Preparing The Antibody
0.01M Na2EDTA 0.3M NaCl, pH 7.5
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
2. Kanda, Y. et al. (2007) Comparison of biological activity among nonfucosylated therapeutic IgG1 antibodies with three different N-linked Fc oligosaccharides: the high-mannose, hybrid, and complex types.Glycobiology. 17 (1): 104-18.
3. Kao, D. et al. (2015) A Monosaccharide Residue Is Sufficient to Maintain Mouse and Human IgG Subclass Activity and Directs IgG Effector Functions to Cellular Fc ReceptorsCell Reports. 6 Dec [Epub ahead of print]