C1q Antibody

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Published customer image:
Sheep anti Human C1q antibody used to evaluate C1q expression by flow cytometry.
Image caption:
Effects of SAP on C1q Binding to S. pneumoniae Measured Using Flow Cytometry. (A–C) Proportion of ST2 (A), ST4 (B), and ST23F (C) S. pneumoniae bacteria positive for C1q after incubation in 90 μg/ml human C1q with or without addition of 10 or 50 μg/ml hSAP. (D) Example of flow cytometry histograms of C1q binding to ST4 S. pneumoniae after incubation in 90 µg/ml human C1q with or without addition of 50 μg/ml hSAP. (E) Proportion of S. pneumoniae positive for C1q after incubation in human serum with (black shading) or without (grey shading) addition of 50 μg/ml hSAP, using C1q-depleted serum (white shading) as a negative control. (F) Example of flow cytometry histograms of C1q binding to ST2 S. pneumoniae after incubation in human serum with or without addition of 50 μg/ml hSAP. For panels (A–C) and (E), error bars represent SDs, and p-values are calculated using 2-tailed t tests.

From: Yuste J, Botto M, Bottoms SE, Brown JS (2007) Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae.
PLoS Pathog 3(9): e120.

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  • Sheep anti Human C1q
  • Sheep anti Human C1q:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP033E, F, IDdatasheet pdfdatasheet pdf1 ml
    AHP033
    AHP033FCdatasheet pdfdatasheet pdf1 ml
    AHP033F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Human C1q antibody recognizes human C1q, a sub-component of the C1 complement component.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human C1q were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.1%EACA
      0.01%Benzamidine
      1mMEDTA
      0.09%Sodium Azide
    • Immunogen
      Human C1q, purified from plasma.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 10 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      negative regulation of macrophage differentiation
      extracellular region
      innate immune response
      negative regulation of granulocyte differentiation
      complement activation, classical pathway
      cell-cell signaling
      complement component C1 complex
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      ImmunodiffusionNeat
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/251/50

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional C1q Antibody Formats

    Formats Applications Sizes available
    C1q Antibody : FITC C 1 ml
    C1q Antibody : Purified E, F, ID 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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