C-Peptide Antibody | 4H8

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  • Mouse anti Human C-Peptide
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  • Product Type
    Monoclonal Antibody
  • Clone
    4H8
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2855Edatasheet pdfdatasheet pdf0.2 mg
    MCA2855
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human C-peptide antibody, clone 4H8 recognizes the human pro-insulin pro-peptide also known as the C-peptide. C-peptide is a 31 amino acid peptide released when proinsulin is cleaved, releasing the α and β insulin chains which form a disulphide linked heterodimer, the active secreted form of insulin within β cells of the islets of Langerhans. When insulin is released from the pancreas into the circulation in response to a rise in serum glucose levels, C-peptide is released in equimolar amounts (Wahren et al. 2000). C-peptide, originally thought to be a biologically inert consequence of insulin processing appears to posess functional qualities, it binds to cell membranes (Rigler et al. 1999) leading to increased intracellular Ca2+ concentration and subsequent stimulation of N+, K+-ATPase and endothelial nitric oxide synthase activities (Zhong et al. 2004). C-peptide also functions in repair of the muscular layer of arteries (Forst and Hunt 2004) and is a potential agent for the treatment of diabetic vasculopathy (Bhatt et al. 2014) . Levels of C-peptide can be used to distinguish between type 1 and type 2 diabetes acting as a biomarker for pancreatic β-cell function, consequently reduced in type 1 diabetes (Kimuni et al. 2014) and may be elevated in type 2 diabetic patients (Oran et al. 2010).

      Mouse anti Human C-Peptide antibody, clone 4H8 recognizes free C-peptide and proinsulin. It does not cross-react with active human, bovine, porcine, mouse or rat insulin and has been successfully employed for immunoprecipitation of human C-peptide prior to MALDI-TOF mass spectrometric analysis (Oran et al. 2010).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional C-Peptide Antibody Formats

    Formats Clone Applications Sizes available
    C-Peptide Antibody : Purified 4H8 E 0.2 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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