c-Jun (pSer73) Antibody

c-Jun (pSer73) Antibody gallery image 1

Western blot analysis of NIH/3T3 cell lysate, treated with (right hand lane) or without (left hand lane) anisomycin, probed with Rabbit anti c-Jun (pSer73) antibody

  • c-Jun (pSer73) Antibody thumbnail image 1
  • Rabbit anti c-Jun (pSer73)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2297P, WB*datasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Rabbit anti c-Jun (pSer73) antibody recognizes proto-oncogene c-Jun, also known as transcription factor AP-1, activator protein 1 (AP1), V-jun avian sarcoma virus 17 oncogene homolog and p39, when phosphorylated at serine 73. The c-Jun transcription factor is encoded by the c-jun oncogene and dimerizes with c-Fos, JDP and ATF family members to form the AP-1 transcription factor. AP-1 is activated by a variety of stimuli including cytokines, stress and growth factors and plays a critical role in cellular processes ranging from proliferation to apoptosis.
      c-Jun becomes phosphorylated on Serine 63 and Serine 73 by MAPK8 (SAPK/JNK) and PLK3, which regulate c-Jun transcription. c-Jun has been implicated in early tumor development, invasion and metastasis and due to these findings has been identified as a target for cancer therapies.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1020 ug/ml
      Western Blotting(1)0.54.0 ug/ml
      For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti c-Jun (pSer 73) recognizes a band of approximately 48 kDa in lysate from NIH3T3 cells treated with the MAP kinase inhibitor anisomycin
    • Instructions For Use

    Additional c-Jun (pSer73) Antibody Formats

    Formats Applications Sizes available
    c-Jun (pSer73) Antibody : Purified P, WB* 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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