Btrc antibody | AB02/4E2
Mouse anti Btrc
- Product Type
- PrecisionAb Monoclonal
- Clone
- AB02/4E2
- Isotype
- IgG1
- Format
- Purified
- Specificity
- Btrc
Antibody Quality and Performance Guarantee
| Mouse anti Human BTRC antibody, clone AB02/4E2 recognizes BTRC, also known as E3RSIkappaB or F-box and WD repeats protein. BTRC encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by BTRC belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6 (provided by RefSeq, Mar 2012). Mouse anti Human BTRC antibody, clone AB02/4E2 detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Mouse anti BTRC antibody detects a band of approximately 55 kDa in HepG2 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Immunogen
- E. coli-derived recombinant human BTRC (aa 52-354)
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q9Y297
- Entrez Gene
- BTRC
- GO Terms
- GO:0005829 cytosol
- GO:0016055 Wnt receptor signaling pathway
- GO:0005634 nucleus
- GO:0016032 viral reproduction
- GO:0019005 SCF ubiquitin ligase complex
- GO:0016566 specific transcriptional repressor activity
- GO:0031145 anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process
- GO:0031648 protein destabilization
- GO:0032582 negative regulation of gene-specific transcription
- View More GO Terms
- GO:0044419 interspecies interaction between organisms
- GO:0045862 positive regulation of proteolysis
- GO:0051437 positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
VMA00649
170517
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