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BRSK1 Antibody

BRSK1 Antibody gallery image 1

Western blot analysis of human brain lysate probed with Rabbit anti Human BRSK1 (AHP1713) at 0.5(A), 1(B) and 2(C) ug/ml

BRSK1 Antibody gallery image 2

Immunohistochemical staining of human brain with Rabbit anti Human BRSK1 (AHP1713)

  • BRSK1 Antibody thumbnail image 1
  • BRSK1 Antibody thumbnail image 2
  • Rabbit anti BRSK1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1713P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti BRSK1 antibody recognizes human BR serine-threonine kinase 1 (BRSK1), a cytoplasmic and nuclear protein belonging to the protein kinase super family.

      BRSK1 is a widely expressed protein, with highest levels being detected in the brain and testis. It is essential for the polarization forebrain neurones which results in the distinct properties of axons and dendrites.

      BRSK1 is involved in the regulation of G2/M arrest and functions as a checkpoint kinase following UV- or methyl methane sulfonate (but not IR-) induced DNA damage. BRSK1 phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo.

      Multiple isoforms of BRSK1 have been identified.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Human BRSK1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to a 28 amino acid sequence from near the centre of Human BRSK1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      neuron differentiation
      response to UV
      G2/M transition DNA damage checkpoint
      protein serine/threonine kinase activity
      protein phosphorylation
      ATP binding
      cell junction
      magnesium ion binding
      establishment of cell polarity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51.0ug/ml
      Immunohistology - Paraffin(1)5.0ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human brain
    • Immunofluorescence
    • Western Blotting
      AHP1713 detects a band of approximately 87kDa in human brain tissue lysate.
    • Instructions For Use

    Additional BRSK1 Antibody Formats

    Formats Applications Sizes available
    BRSK1 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Histar Detection SystemSTAR3000A50 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human brain
                • Product Images

                • BRSK1 Antibody thumbnail image 1
                • BRSK1 Antibody thumbnail image 2

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