Rabbit anti BRSK1 antibody recognizes human BR serine-threonine kinase 1 (BRSK1), a cytoplasmic and nuclear protein belonging to the protein kinase super family.
BRSK1 is a widely expressed protein, with highest levels being detected in the brain and testis. It is essential for the polarization forebrain neurones which results in the distinct properties of axons and dendrites.
BRSK1 is involved in the regulation of G2/M arrest and functions as a checkpoint kinase following UV- or methyl methane sulfonate (but not IR-) induced DNA damage. BRSK1 phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo.
Multiple isoforms of BRSK1 have been identified.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antisera to Human BRSK1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
0.02% Sodium Azide (NaN3)
A peptide corresponding to a 28 amino acid sequence from near the centre of Human BRSK1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1713 detects a band of approximately 87kDa in human brain tissue lysate.