BRCA1 Antibody | MS13

BRCA1 Antibody | MS13 gallery image 1

Published customer image:
Muse antiHuman BRCA1 antibody, clone MS13 (MCA1683GT) used for the demonstration of BRCA1 expressing tumor cells by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Cytoplasm staining of BRCA1 in neoplastic cells from an oral squamous cell carcinoma (OSCC). (B) Nuclear staining of RAD51 in neoplastic cells from an OSCC. (C) Nuclear staining of p53 in OSCC cells. (D) Nuclear staining of ?H2AX in OSCC cells (original magnification ×200 in all photomicrographs).

From: Oliveira-Costa JP, Oliveira LR, Zanetti R, Zanetti JS, da Silveira GG, Chavichiolli Buim ME, Zucoloto S, Ribeiro-Silva A, Soares FA.
BRCA1 and γH2AX as independent prognostic markers in oral squamous cell carcinoma.
Oncoscience. 2014 Jun 1;1(5):383-91.

  • BRCA1 Antibody | MS13 thumbnail image 1
  • Mouse anti Human BRCA1 (N-Terminal)
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1683GTIF, IP, P, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Mouse anti Human BRCA1 antibody, clone MS13 recognizes the human tumor supressor protein BRCA-1, also known as Breast cancer type 1 susceptibility protein or RING finger protein 53. BRCA1 is a 1863 amino acid, ~220kDa E3 ubiquitin-protein ligase playing a central role in DNA repair (Tibbetts et al. 2000), expressed in the nucleus during the S/G2 phase of the cell cycle (Durrant and Nickoloff 2005). Normal BRCA-1 acts as a tumor supressor protein and mutation or dysregulation of BRCA1 may indicate high risk of development of disease, including breast cancer (Budhram-Mahadeo et al. 1999). Mouse anti human BRCA-1, clone MS13 recognizes an epitope within the 304 amino acid N-Terminal (NT) region (Yoshikawa et al. 1999) of human BRCA1.

      BRCA1 is expressed numerous organs including mammary and ovarian tissues (Miki et al. 1994) . Mutations in the BRCA-1 gene are associated with hereditary breast and ovarian cancers, particularly at a younger age of diagnosis. Women carrying BRCA1 mutations have a 50-95% chance of developing breast cancer in later life (Elit 2001), secondary cancers such as prostate or melanoma also frequently arise in the latter stages of both male and female breast cancer patients (Benevento et al. 2012), but genetic screening and increased awareness of preventative surgery, can reduce this risk significantly (Scheuer et al. 2002). However, in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations (Gallagher et al. 2010).

      BRCA1 is a key marker of triple-negative breast cancer (TNBC), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and lacks the benefit of specific targeted therapy (Duffy et al. 2012). Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women (Liu et al. 2009).

      Studies looking at sporadic breast cancer, have shown that clone MS13 strongly correlates with poor patient prognosis, and appears to label the Δ11b splice variant of BRCA1, suggesting that Δ11b could act as a negative marker in the prognosis of sporadic breast cancer (Fraser et al. 2003) Mouse anti human BRCA1 antibody, clone MS13 is suitable for use in the immunohistochemical staining of human breast (Fraser et al. 2003) and for immunofluorescence in multiple cell lines (Scully et al. 1996).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/50
      Western Blotting0.5ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Breast carcinoma
    • Immunofluorescence
    • Western Blotting
      Mouse anti Human BRCA1 detects a band of approximately 220kDa in HeLa nuclear extract. Some cell lysates may also show an uncharacterized band of approximately 65kDa.
    • Instructions For Use

    Additional BRCA1 Antibody Formats

    Formats Clone Applications Sizes available
    BRCA1 Antibody : Purified MS13 IF, IP, P, WB 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Breast carcinoma


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