BRCA1 Antibody | 8F7

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  • Mouse anti Human BRCA1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    8F7
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5947GTC *, IP, P *, WBdatasheet pdfdatasheet pdf50 µg
    MCA5947GT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human BRCA1, clone 8F7 recognises human BRCA1, otherwise known as breast cancer type 1 susceptibility protein, a tumor suppressor gene and major player in DNA damage repair, predominantly expressed in the nucleus during the S/G2 phase of the cell cycle.

      Along with BRCA2, BRCA1 is a high risk gene which is associated with hereditary breast and ovarian cancers, particularly at a younger age of diagnosis. Women carrying the BRCA1 mutation have a 50-95% chance of developing breast cancer in later life, but genetic screening and increased awareness of preventative surgery, can reduce this risk significantly. Deleterious BRCA1 mutations may also increase the risk of other cancers in both males and females including pancreatic cancer, although in males pancreatic and prostate cancer appear to be more strongly associated with BRCA2 gene mutations.

      BRCA1 is a key marker of triple-negative breast cancer/TNBC (ER-/PR-/HER2-), a high risk aggressive cancer which makes up about 15% of invasive breast cancers, and which lacks the benefit of specific therapy that targets the three major proteins ER/PR/HER2. Triple-negative tumors are predominantly basal-like, poorly differentiated and of higher histological grade. Younger women have an increased rate of basal or BRCA related TNBC, compared with the higher proportion of apocrine, normal-like and rare subtypes of TNBC, seen in older women.

      Mouse anti BRCA1, clone 8F7 recognises full length 220kDa BRCA1, and is suitable for use in immunohistochemical studies of both frozen and paraffin sections see Yoshikawa, K. et al. for details. .

      Several studies have shown that clone 8F7 exhibits strong and highly specific nuclear staining.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoprecipitation
      Western Blotting1.010.0ug/ml
      Immunohistology - Frozen(1)
      Immunohistology - Paraffin(2)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of frozen sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.
      (2)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Breast carcinoma
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional BRCA1 Antibody Formats

    Formats Clone Applications Sizes available
    BRCA1 Antibody : Purified 8F7 C *, IP, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Breast carcinoma

              References

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