BNIP3L Antibody

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BNIP3L Antibody gallery image 1

Western blot analysis of whole cell lysates from K562 erythromyeloblastoid cells probed with Rabbit anti BNIP3L (AHP1885) in the absence (A) or presence (B) of blocking peptide

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BNIP3L Antibody gallery image 2

Immunohistochemical staining of human kidney with Rabbit anti Human BNIP3L (AHP1885)

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  • BNIP3L Antibody thumbnail image 1
  • BNIP3L Antibody thumbnail image 2
  • Rabbit anti Human BNIP3L
  • Rabbit anti Human BNIP3L
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1885P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1885
    AHP1885TP *, WBdatasheet pdfdatasheet pdf50 µg
    AHP1885T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human BNIP3L antibody recognizes human BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L), a 299 aa single-pass membrane protein located on the nuclear envelope and endoplasmic recticulum.

      BNIP3L is interacts with viral proteins and cellular anti apoptosis proteins inducing apoptosis. BNIP3L also inhibits apoptosis induced by BNIP3.

      BNIP3t may function as a tumour suppressor.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human BNIP3L was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to the amino acids sequence 77 - 92 of human BNIP3L.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      protein heterodimerization activity
      integral to membrane
      negative regulation of survival gene product expression
      interspecies interaction between organisms
      nuclear envelope
      endoplasmic reticulum
      defense response to virus
      mitochondrial envelope
      induction of apoptosis
      apoptosis
      protein homodimerization activity
      lamin binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1ug/ml
      Immunohistology - Paraffin(1)2ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1ug/ml
      Immunohistology - Paraffin(1)2ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human kidney
    • Histology Positive Control Tissue
      Human kidney
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AHP1885 detects a bands of approximately 36 and 40kDa in K562 cell lysate.
    • Western Blotting
      AHP1885T detects a bands of approximately 36 and 40kDa in K562 cell lysate.
    • Instructions For Use
    • Instructions For Use

    Additional BNIP3L Antibody Formats

    Formats Applications Sizes available
    BNIP3L Antibody : Purified P *, WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Human kidney
              Human kidney

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