Bone morphogenetic protein 9 (BMP-9), otherwise known as growth differentiation factor-2 (GDF-2), plays a role in the response of embryonic basal forebrain cholinergic neurons (BFCN) to the neurotransmitter acetylcholine, and hence in memory and learning processes. BMP-9 is predominantly expressed in liver nonparenchymal cells, and has several biological properties, including induction of orthotopic bone formation, glucose homeostasis, iron homeostasis, and in the inhibition of angiogenesis.
Purified recombinant protein - lyophilised
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum albumin (BSA) is recommended.
Purified recombinant BMP-9 expressed in CHO cells
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
24.1kD (110 amino acid residue homodimer)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.