PHP168 is a 28.8 kDa recombinant protein corresponding to amino acid residues 316 to 431 of the full-length human bone morphogenetic protein 7 (BMP-7) precursor.
BMP-7, also known as Osteogenic protein 1 (OP-1), is a disulphide-linked homodimeric cysteine knot protein and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.
Purified recombinant protein - lyophilised
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant BMP-7 expressed in CHO cells.
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 ug/ml.
>98% by SDS PAGE/HPLC analysis
Approx. Protein Concentrations
Total protein concentration 0.1mg/ml after reconstitution.
Reagents In The Kit
Preparing The Antibody
Lyophilised from 0.1% TFA
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
PHP168 may be used as a standard in a sandwich ELISA with AHP961 as the capture reagent and AHP961B as the detection antibody.
Histology Positive Control Tissue
PHP168 may be used as a positive control for Western blotting applications with AHP961 or AHP961B.