BMP-7 Antibody | M1-F8

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BMP-7 Antibody | M1-F8 gallery image 1

Western blot analysis of BMP-7 expression in Raw 2647 using Mouse anti Human BMP-7:Azide Free (MCA2968Z)

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BMP-7 Antibody | M1-F8 gallery image 2

Western blot analysis of BMP-7 expression in human uterus myoma using Mouse anti Human BMP-7:Azide Free (MCA2968Z)

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BMP-7 Antibody | M1-F8 gallery image 3

Published customer image:
Mouse anti Human BMP-7 antibody, clone M1-F8 (MCA2968Z) used for the evaluation of BMP-7 protein expression levels in different cell types by western blotting
Image caption:
Normal and malignant B cells express BMP6 and BMP7.
BMP mRNA expression was determined by real-time RT-PCR, and the results are relative to the expression in human fetal brain tissue. (A) Centrocytes (CD19+CD38+IgD-CD77-) or centroblasts (CD19+CD38+IgD-CD77+) from normal human tonsils (relative expression ± SD of duplicates). (B) Lymphoma cell lines (means ± SEM, n=3 for BMP6; n=5 for BMP7). (C) Purified malignant B cells (CD20+CD10+Igκ/Igλ+) or tumor-infiltrating T cells (CD20-CD3+) from lymphoma patient samples (relative expression ± SD of duplicates). (D) Relative expression of BMP6 and BMP7 across non-Hodgkin's lymphoma (NHL; left of line) and in normal B-cell populations (right of line) obtained from gene expression data set [16]. (E-F) Protein expression of BMP-7 was determined by Western blot analysis. Protein bands correspond to propeptide (lower band) and uncleaved full-length monomer (upper band). HeLa_BMP-7: Lysate from HeLa cells transfected with BMP-7. β-tubulin was used as loading control. One representative experiment (E) and mean values (F) of BMP-7 levels are shown (means ± SEM, n=3).

From: Huse K, Bakkebø M, Wälchli S, Oksvold MP, Hilden VI, Forfang L, et al. (2012)
Role of Smad Proteins in Resistance to BMP-Induced Growth Inhibition in B-Cell Lymphoma.
PLoS ONE 7(10): e46117.

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  • Mouse anti Human BMP-7:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    M1-F8
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2968ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA2968Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human BMP-7 antibody, clone M1-F8 recognizes human Bone morphogenetic protein 7, also known as BMP-7, Osteogenic protein 1 or Eptotermin alfa. BMP-7 is a 139 amino acid active peptide with an additional 29 amino acid signal peptide and a 263 propeptide region. The holoprotein is a glycoprotein of ~49 kDa involved in the positive regulation of bone and cartilage synthesis.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional BMP-7 Antibody Formats

    Formats Clone Applications Sizes available
    BMP-7 Antibody : Preservative Free M1-F8 WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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