BMP-7 Antibody

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BMP-7 Antibody gallery image 1

Formalin fixed, paraffin embedded human breast invasive ductal carcinoma stained with Rabbit anti Human BMP-7 following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)

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  • BMP-7 Antibody thumbnail image 1
  • Rabbit anti Human BMP-7
  • Rabbit anti Human BMP-7:Biotin
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP961E, P *, WBdatasheet pdfdatasheet pdf50 µg
    AHP961
    AHP961BE, WBdatasheet pdfdatasheet pdf50 µg
    AHP961B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human BMP-7 antibody recognizes the disulphide-linked homodimeric cysteine knot protein known as human Bone Morphogenetic Protein 7 (BMP-7) or Osteogenic protein 1 (OP-1). BMP-7 is a member of the transforming growth factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation, and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis.

      BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation.

      Studies into the properties of BMP-7 have largely focused on its role during ontogeny of the kidney, owing to its prominent expression in the renal tubules. Results from research into experimental diabetic nephropathy have indicated that BMP-7 has antifibrogenic properties and appears to act as an antagonist to TGF-Beta (3-4).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

      For long term storage the addition of 0.09% sodium azide is recommended.
    • Preparation
    • Antiserum Preparation
      Antisera to human BMP-7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      None present
    • Immunogen
      Recombinant human BMP-7.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
      cartilage development
      negative regulation of mitosis
      BMP signaling pathway
      negative regulation of phosphorylation
      negative regulation of gene-specific transcription
      cytokine activity
      mesonephros development
      epithelial to mesenchymal transition
      protein localization to nucleus
      positive regulation of bone mineralization
      steroid hormone mediated signaling pathway
      positive regulation of pathway-restricted SMAD protein phosphorylation
      growth
      pathway-restricted SMAD protein phosphorylation
      negative regulation of glomerular mesangial cell proliferation
      negative regulation of MAP kinase activity
      SMAD protein signal transduction
      ossification
      ureteric bud development
      growth factor activity
      positive regulation of osteoblast differentiation
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.251.0ug/ml
      Western Blotting0.10.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      AHP961 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with AHP961B as the detection antibody and PHP168 as the standard.
    • ELISA
      AHP961B may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AHP961 as the capture antibody and PHP168 as the standard.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AHP961 may be used in Western blotting with PHP168 as the positive control.
    • Western Blotting
      AHP961B may be used in Western blotting with PHP168 as the positive control.
    • Instructions For Use
    • Instructions For Use

    Additional BMP-7 Antibody Formats

    Formats Applications Sizes available
    BMP-7 Antibody : Biotin E, WB 50 µg
    BMP-7 Antibody : Purified E, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
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      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
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      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Rabbit anti Human BMP-7:BiotinAHP961B50 µgE, WB
          AHP961B
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A
          Recombinant Human BMP-7PHP16810 µgE, FN, WB
          PHP168
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Recombinant Human BMP-7PHP16810 µgE, FN, WB
          PHP168

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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