BMP-4 Antibody (PrecisionAbTM Antibody)

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BMP-4 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with BMP-4 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 8 second exposure. Arrow points to BMP-4 (molecular weight 47 kDa)

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  • BMP-4 Antibody with Control Lysate
  • BMP-4 Antibody with Control Lysate
  • Rabbit anti BMP-4
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    Rabbit anti human BMP-4 antibody recognizes bone morphogenetic protein 4, also known as BMP4 or bone morphogenetic protein 2B (BMP-2B). This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00302WBdatasheet pdfdatasheet pdf100 µl
      VPA00302
      VPA00302KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00302K
      VPA00302KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00302KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human BMP-4 antibody recognizes bone morphogenetic protein 4 (BMP-4) also known as bone morphogenetic protein 2B (BMP-2B).

        BMP-4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo at an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of the BMP-4 gene sequence has been described and three variants reported, all encoding an identical protein (provided by RefSeq, Jul 2008).

        Rabbit anti Human BMP-4 antibody detects a band of 47 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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