BMP-3

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  • Recombinant Human BMP-3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP246FNdatasheet pdfdatasheet pdf50 µg
    PHP246
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Bone morphogenetic protein 3 (BMP-3), otherwise known as Osteogenin, is highly expressed in adult bone, especially chondroblasts, osteoblasts and osteoclasts, and, like other BMPs, induces bone and cartilage development. BMP-3 acts as an inhibitor of osteogenesis and bone formation, through the activation of a signalling cascade, which antagonizes pro-osteogenic BMP signalling. PHP246 corresponds to residues 361 to 472 of the 472 amino acid BMP-3 precursor protein.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 500ul distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant BMP-3 expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      Determined by its ability to inhibit BMP-2-induced alkaline phosphatase production by ATDC-5 cells.
    • Purity
      >95% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
      0.1% Trifluoroacetic acid
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      ossification
      growth
      growth factor activity
      cartilage development
      extracellular space
      cell differentiation
      BMP receptor binding
      cytokine activity
      cell-cell signaling
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional BMP-3 Formats

    Formats Applications Sizes available
    BMP-3 : Rec. Protein FN 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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