Recombinant Bone morphogenetic protein 3 (BMP-3), otherwise known as Osteogenin, is highly expressed in adult bone, especially chondroblasts, osteoblasts and osteoclasts, and, like other BMPs, induces bone and cartilage development. BMP-3 acts as an inhibitor of osteogenesis and bone formation, through the activation of a signalling cascade, which antagonizes pro-osteogenic BMP signalling. Recombinant Bone morphogenetic protein 3 (PHP246) corresponds to residues 361 to 472 of the 472 amino acid BMP-3 precursor protein.
Purified recombinant protein - lyophilised
Reconstitute with 500ul distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Purified recombinant BMP-3 expressed in E.coli
Determined by its ability to inhibit BMP-2-induced alkaline phosphatase production by ATDC-5 cells.
>95% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Reagents In The Kit
Preparing The Antibody
0.1% Trifluoroacetic acid
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.