BLTR Antibody | 202/7B1

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BLTR Antibody | 202/7B1 gallery image 1

Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:Alexa Fluor® 647 (MCA2108A647)

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BLTR Antibody | 202/7B1 gallery image 2

Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:FITC (MCA2108F)

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BLTR Antibody | 202/7B1 gallery image 3

Staining of human peripheral blood granulocytes with Mouse anti Human BLTR:RPE (MCA2108PE)

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BLTR Antibody | 202/7B1 gallery image 4

Published customer image:
Mouse anti Human BLTR antibody, clone 202/7B1 used for the detection of Leukotriene B4 receptor 1 expressing cultured peripheral blood mononuclear cells by immunofluorescence.
Image caption:
Leukotriene B4 biosynthetic enzymes and receptors are expressed in both macrophages and osteoclasts. (a) Quantification of leukotriene B4 (LTB4) levels by enzyme-linked immunoassay in the conditioned medium of human peripheral blood mononuclear cells (PBMCs) treated with 25 ng/ml macrophage colony-stimulating factor (M-CSF) and 10 ng/ml interleukin 23 (IL-23) for 3 days. Representative data from three experiments are shown. **P < 0.01. (b) Immunofluorescence of human PBMCs cultured with either M-CSF alone for 8 days or M-CSF + receptor activator of nuclear factor ?B ligand (RANKL) for 14 days showing the expression of BLT1, BLT2, 5-lipoxygenase (5-LO) and leukotriene A4 hydrolase (LTA4H) in mononuclear and multinucleated giant cells. Representative images from three experiments are shown. 4',6-diamidino-2-phenylindole (DAPI) is blue and BLT1, BLT2, 5-LO and LTA4H are in red. The scale bars represent 20 µm in Figure 1b.

From:Dixit N, Wu DJ, Belgacem YH, Borodinsky LN, Gershwin M, Adamopoulos IE. Leukotriene B4 activates intracellular calcium and augments human osteoclastogenesis. Arthritis Res Ther. 2014 Dec 2;16(6):496.

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BLTR Antibody | 202/7B1 gallery image 5

Staining of human peripheral blood monocytes with Mouse anti Human BLTR (MCA2108GA)

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BLTR Antibody | 202/7B1 gallery image 6

Staining of human peripheral blood monocytes with Mouse anti Human BLTR: Azide Free (MCA2108XZ)

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  • BLTR Antibody | 202/7B1 thumbnail image 1
  • BLTR Antibody | 202/7B1 thumbnail image 2
  • BLTR Antibody | 202/7B1 thumbnail image 3
  • BLTR Antibody | 202/7B1 thumbnail image 4
  • BLTR Antibody | 202/7B1 thumbnail image 5
  • BLTR Antibody | 202/7B1 thumbnail image 6
  • Mouse anti Human BLTR:FITC
  • Mouse anti Human BLTR:RPE
  • Mouse anti Human BLTR:Alexa Fluor® 647
  • Mouse anti Human BLTR:FITC
  • Mouse anti Human BLTR
  • Mouse anti Human BLTR
  • Mouse anti Human BLTR:Alexa Fluor® 647
  • Mouse anti Human BLTR
  • Mouse anti Human BLTR:RPE
  • Mouse anti Human BLTR:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    202/7B1
  • Isotype
    IgG2a
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2108FC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA2108F
    MCA2108GAC, F, IF, IYdatasheet pdfdatasheet pdf0.1 mg
    MCA2108GA
    MCA2108C, F, IF, IYdatasheet pdfdatasheet pdf0.2 mg
    MCA2108
    MCA2108XZC, F, IF, IYdatasheet pdfdatasheet pdf1 mg
    MCA2108XZ
    MCA2108PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2108PE
    MCA2108A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2108A647
    MCA2108FTC, Fdatasheet pdfdatasheet pdf25 µg
    MCA2108FT
    MCA2108TC, F, IF, IYdatasheet pdfdatasheet pdf25 µg
    MCA2108T
    MCA2108PETFdatasheet pdfdatasheet pdf25 Tests
    MCA2108PET
    MCA2108A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2108A647T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human BLTR antibody, clone 202/7B1 recognizes Human leukotriene B4 receptor, also known as BLTR, Chemoattractant receptor-like 1, G-protein coupled receptor 16 or P2Y purinoceptor 7. BLTR is a 353 ~40 kDa multipass transmembrane glycoprotein

      BLTR is expressed by peripheral blood granulocytes and is important in the pro-inflammatory response.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%
      1%
      Sodium Azide
      Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%
      1%
      Sodium Azide
      Bovine Serum Albumin
      0.09%
      1%
      Sodium Azide
      Bovine Serum Albumin
      Pack Size: 0.1 mg, 25 µg
      0.09%Sodium Azide
      Pack Size: 0.2 mg0.09% sodium azide.
      Pack Size: 0.1 mg, 25 µg
      0.09%Sodium Azide
      Pack Size: 0.2 mg0.09% sodium azide.
      0.09%
      1%
      Sodium Azide
      Bovine Serum Albumin
      Pack Size: 0.1 mg, 25 µg
      0.09%Sodium Azide
      Pack Size: 0.2 mg0.09% sodium azide.
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      None present
    • Immunogen
      HeLa cell transfectants expressing human BLTR.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger
      cellular component movement
      inflammatory response
      immune response
      muscle contraction
      nucleotide binding
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/2
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 0.2 mg, 25 µg
      1/100 Pack Size: 0.1 mg
      1/100Pack Size: 0.2 mg, 25 µg
      1/200Pack Size: 0.1 mg
      Immunoassay
      Immunofluorescence
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 0.2 mg, 25 µg
      1/100 Pack Size: 0.1 mg
      1/100Pack Size: 0.2 mg, 25 µg
      1/200Pack Size: 0.1 mg
      Immunoassay
      Immunofluorescence
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 0.2 mg, 25 µg
      1/100 Pack Size: 0.1 mg
      1/100Pack Size: 0.2 mg, 25 µg
      1/200Pack Size: 0.1 mg
      Immunoassay
      Immunofluorescence
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/2
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Immunoassay
      Immunofluorescence
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional BLTR Antibody Formats

    Formats Clone Applications Sizes available
    BLTR Antibody : Purified 202/7B1 C, F, IF, IY 0.2 mg | 25 µg | 0.1 mg
    BLTR Antibody : Alexa Fluor® 647 202/7B1 F 100 Tests/1ml | 25 Tests/0.25ml
    BLTR Antibody : Preservative Free 202/7B1 C, F, IF, IY 1 mg
    BLTR Antibody : RPE 202/7B1 F 25 Tests | 100 Tests
    BLTR Antibody : FITC 202/7B1 C, F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:RPEMCA929PE100 TestsF
        MCA929PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:Alexa Fluor® 647MCA929A647100 Tests/1mlF
        MCA929A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:Alexa Fluor® 647MCA929A647100 Tests/1mlF
        MCA929A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:RPEMCA929PE100 TestsF
        MCA929PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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