BETA-III Spectrin Antibody (PrecisionAbTM Antibody)

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BETA-III Spectrin Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with beta-III spectrin antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 4 second exposure. Arrow points to beta-III spectrin (molecular weight 270 kDa)

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  • BETA-III Spectrin Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • BETA-III Spectrin Antibody with Control Lysate
  • Mouse anti BETA-III Spectrin
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    Beta-III spectring antibody recognizes the beta-III spectrin, also known as SCA5.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2b
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00694WBdatasheet pdfdatasheet pdf100 µl
      VMA00694
      VMA00694KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00694KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human beta-III spectrin antibody recognizes the beta-III spectrin, also known as SCA5.

        Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by SPTBN2 (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane. Mutations in SPTBN2 cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements (provided by RefSeq, Dec 2009).

        Mouse anti Human beta-III spectrin antibody detects a band of 270 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti beta-III spectrin detects a band of approximately 270 kDa in HepG2 cell lysates
        Mouse anti beta-III spectrin detects a band of approximately 270 kDa in HepG2 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - Liquid
        Purified IgG - Liquid
      • Preparation
        20μl Mouse monoclonal antibody affinity purified on Protein A from tissue culture supernatant
        Mouse monoclonal antibody affinity purified on Protein A from tissue culture supernatant
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide
        0.09% Sodium Azide
      • Immunogen
        Recombinant protein of partial human beta-III spectrin (amino acids 356-475)
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20°C, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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