Beta Arrestin-2 Antibody

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Human spleen probed with Goat anti human Beta arrestin-2 (AHP2038)

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Western blotting analysis of Human Brain lysate (35μg protein in RIPA buffer) probed with Goat anti Human beta arrestin-2 antibody (AHP2038) at 0.3μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

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Immunohistochemical staining of formalin fixed, paraffin embedded human cerebellum probed with Goat anti Human beta arrestin-2 antibody (AHP2038) at 4μgml-1 following heat mediated antigen retrieval with citrate buffer pH 6.0

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  • Goat anti Human Beta Arrestin-2
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2038E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human beta arrestin-2 antibody recognises human beta arrestin-2, a 409 amino acid ~45 kDa scaffold protein and member of the arrestin family, highly expressed in the central nervous system. Beta-arrestin-2 plays a role in regulating agonist-mediated G-protein coupled receptor (GPCR) signaling, through receptor desensitization and internalization. Beta-arrestin-2 may function in the regulation of synaptic receptors, and in scaffold-linking GPCRs to JNK3, thereby enhancing the GPCR-mediated activation of JNK3, associated with cell apoptosis.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human Beta arrestin-2 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-HDHIPLPRPQS from the internal region of the ARRB2 near the C-terminus (aa 350-360) as indicated in UniProt entry: P32121.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.030.1ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP2038 detects a band of approximately 45kDa in human brain, skin and spleen cell lysates.
    • Instructions For Use

    Additional Beta Arrestin-2 Antibody Formats

    Formats Applications Sizes available
    Beta Arrestin-2 Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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