Beta 2 Microglobulin Antibody (PrecisionAbTM Antibody)

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Beta 2 Microglobulin Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with beta 2 microglobulin antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 166 second exposure, Arrow points to beta 2 microglobulin (molecular weight 12 kDa)

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  • Beta 2 Microglobulin Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti Beta 2 Microglobulin
  • Beta 2 Microglobulin Antibody with Control Lysate
  • Beta 2 Microglobulin Antibody with Control Lysate
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    beta 2 microglobulin antibody recognizes beta 2 microglobulin. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00003WBdatasheet pdfdatasheet pdf100 µl
      VMA00003
      VMA00003KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00003K
      VMA00003KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00003KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human beta 2 microglobulin antibody recognizes native human beta 2 microglobulin, a 119 amino acid component of MHC class I molecules present on all nucleated cells, containing a single Ig-like C1 type domain. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients (Woo et al. 2014). Also known as B2M, it has been suggested that this protein can be used as a prognostic marker for HIV infection (Halota et al. 2002).

        Mouse anti Human beta 2 microglobulin antibody recognizes β2 microglobulin as a band of ~12 kDa in HeLa cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti beta 2 microglobulin antibody detects a band of approximately 12 kDa in HeLa cell lysates.
        Anti beta 2 microglobulin antibody detects a band of approximately 12 kDa in HeLa cell lysates.
        Anti beta 2 microglobulin antibody detects a band of approximately 12 kDa in HeLa cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography on Protein A from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        5% Glycerol
        0.09% Sodium Azide (NaN3)
        5% Glycerol
        0.09% Sodium Azide (NaN3)
        5% Glycerol
      • Immunogen
        Highly pure human beta 2 microglobulin from urine
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        Golgi membrane
        antigen processing and presentation of peptide antigen via MHC class I
        interferon-gamma-mediated signaling pathway
        early endosome membrane
        immune response
        MHC class I protein complex
        viral reproduction
        protein binding
        regulation of defense response to virus by virus
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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