Beta 2 Microglobulin Antibody | 3F9-2C2

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Beta 2 Microglobulin Antibody | 3F9-2C2 gallery image 1

Staining of paraffin embedded human esophagus with Mouse anti Human β2 microglobulin antibody (MCA3992Z)

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Beta 2 Microglobulin Antibody | 3F9-2C2 gallery image 2

Immunofluorescence showing β2 microglobulin in HeLa human adenocarcinoma cells using Mouse anti Human β2 microglobulin antibody (MCA3992Z)

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Beta 2 Microglobulin Antibody | 3F9-2C2 gallery image 3

Western blot analysis of β2 microglobulin expression in U-2 human osteosarcoma whole cell lysate using Mouse anti Human β2 microglobulin antibody (MCA3992Z) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence

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Beta 2 Microglobulin Antibody | 3F9-2C2 gallery image 4

Western blot analysis of HeLa human adenocarcinoma whole cell lysate probed with Mouse anti Human β2 microglobulin antibody (MCA3992Z) followed by HRP conjugated Goat anti Mouse IgG, visualized using chemiluminescence

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  • Mouse anti Human Beta 2 Microglobulin:Preservative Free
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  • Product Type
    Monoclonal Antibody
  • Clone
    3F9-2C2
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3992ZIF, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA3992Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human beta 2 Microglobulin antibody, clone 3F9-2C2 recognizes native human beta 2 microglobulin, a 119 amino acid pro-peptide cleaved of it's signal peptide to yield the 99 amino acid mature form of ~12 kDa with a pI of 5.6, containing a single C1 type Ig-like domain with multiple potentilal N-glycosylation sites. β2 microglobulin is a component of MHC class I system forming heterodimers with the α chain of MHC class I molecules and CD1. MHC Class I - β2 microglobulin heterodimers are responsible for presentation of peptide antigens to the immune system and are which present on all nucleated cells. β2 microglobulin is also involved in immunoglobulin transport (Story et al. 1994) and iron metabolism (Santos et al. 1996).

      Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients where it may take on a fibrillar multimeric form contributing to amyloidosis in chronic renal dialysis patients (Gorevic et al. 1985) . It has been suggested that this protein can be used as a prognostic marker for HIV infection (Franzetti et al. 1988). β2 microglobulin may also be a marker for the progression of multiple myeloma (Rossi et al. 2010).

      Mouse anti Human β2 microglobulin antibody, clone 3F9-2C2 havs been used successfully for the detection of β2 microglobulin in human tissues and celll lines using both immunohistochemistry and Western blotting.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present.
    • Immunogen
      Recombinant protein corresponding to amino acids 1 - 120 of human beta 2 microglobulin.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      Golgi membrane
      regulation of defense response to virus by virus
      protein binding
      antigen processing and presentation of peptide antigen via MHC class I
      early endosome membrane
      viral reproduction
      interferon-gamma-mediated signaling pathway
      immune response
      MHC class I protein complex
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence0.110 ug/ml
      Western Blotting
      Immunohistology - Paraffin(1)0.110 ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human oesophagus.
    • Immunofluorescence
    • Western Blotting
      Mouse anti human &beta-2-microglobulin antibody, clone 3F9-2C2 detects a band of ~12 kDa in U-2 OS cell lysates
    • Instructions For Use

    Additional Beta 2 Microglobulin Antibody Formats

    Formats Clone Applications Sizes available
    Beta 2 Microglobulin Antibody : Preservative Free 3F9-2C2 IF, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
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      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human oesophagus.

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