BETA-1 Adrenergic Receptor Antibody (PrecisionAbTM Antibody)

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BETA-1 Adrenergic Receptor Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with beta-1 adrenergic receptor antibody followed by detection with Goat anti Rabbit HRP conjugated (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to beta-1 adrenergic receptor (molecular weight 50-55 kDa)

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  • BETA-1 Adrenergic Receptor Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • BETA-1 Adrenergic Receptor Antibody with Control Lysate
  • Rabbit anti BETA-1 Adrenergic Receptor
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    Beta-1 adrenergic receptor antibody recognizes the beta-1 adrenergic receptor, also known as RHR, ADRB1, or BETA1AR .
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00859WBdatasheet pdfdatasheet pdf100 µl
      VPA00859
      VPA00859KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00859KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human beta-1 adrenergic receptor antibody recognizes the beta-1 adrenergic receptor, also known as RHR, ADRB1 and BETA1AR .

        The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in ADRB1 have been shown to affect the resting heart rate and can be involved in heart failure (provided by RefSeq, Jul 2008).

        Rabbit anti Human beta-1 adrenergic receptor antibody detects bands of 50-55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Anti beta-1 adrenergic receptor antibody recognizes a band of approximately 50-55 kDa in HEK293 cell lysates
        Anti beta-1 adrenergic receptor antibody recognizes a band of approximately 50-55 kDa in HEK293 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        20μl Rabbit polyclonal antibody purified by affinity chromatography on Protein A
        Rabbit polyclonal antibody purified by affinity chromatography on Protein A
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide
        2% Sucrose
        0.09% Sodium Azide
        2% Sucrose
      • Immunogen
        Synthetic peptide corresponding to the C-terminal region of human beta-1 adrenergic receptor
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20°C, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        protein heterodimerization activity
        integral to plasma membrane
        alpha-2A adrenergic receptor binding
        beta1-adrenergic receptor activity
        positive regulation of cAMP biosynthetic process
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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